Ishii, et al. (2001): Increase of oligodendrocyte progenitor cells after spinal cord injury

Wise Young · 09-21-2001, 05:51 AM

• Ishii K, Toda M, Nakai Y, Asou H, Watanabe M, Nakamura M, Yato Y, Fujimura Y, Kawakami Y, Toyama Y and Uyemura K (2001). Increase of oligodendrocyte progenitor cells after spinal cord injury. J Neurosci Res. 65 (6): 500-7. Summary: The reaction of oligodendrocyte progenitor cells (OPCs) after spinal cord injury (SCI) is poorly understood. In this study, we examined oligodendroglial reactions after contusion SCI in adult rats by immunohistochemistry. OPCs were identified by staining with monoclonal antibodies (mAbs) A2B5 and O4. Each of the A2B5-, O4-positive OPCs and galactocerebroside-positive oligodendrocytes dramatically increased in the lesion of the dorsal posterior funiculus. Bromodeoxyuridine (BrdU) incorporation studies showed that most O4-positive cells in the lesion were labeled with BrdU, suggesting that these OPCs were proliferative. In contrast, the expression of myelin basic protein was decreased in the lesion compared with controls that received laminectomy only. From the injured cord, OPCs were isolated by immunopanning with mAb A2B5. We observed an increased number of OPCs from the injured spinal cords compared with those isolated from controls and unoperated animals. After several days in culture, the OPCs from the lesion expressed galactocerebroside. These results suggest that OPCs are induced and can differentiate following SCI in the adult rat. Copyright 2001 Wiley- Liss, Inc. <http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?db=m&form=6&dopt=r&uid=11550218> Department of Orthopaedic Surgery, Keio University School of Medicine, Shinjuku-ku, Tokyo.

[This message was edited by Wise Young on September 23, 2001 at 10:13 PM.]

Wise Young · 09-21-2001, 09:57 PM

This paper confirms what many scientists have long speculated about. Injury causes a stem cells to produce oligodendroglial precursor cells (OPCs) that then produce oligodendroglial cells that remyelinate the spinal cord. This is true of the rat and presumably also true of humans. Wise.

09-21-2001, 05:51 AM	#1
Wise Young Administrator Join Date: Jul 2001 Location: New Brunswick, NJ, USA Posts: 34,097	Ishii, et al. (2001): Increase of oligodendrocyte progenitor cells after spinal cord injury • Ishii K, Toda M, Nakai Y, Asou H, Watanabe M, Nakamura M, Yato Y, Fujimura Y, Kawakami Y, Toyama Y and Uyemura K (2001). Increase of oligodendrocyte progenitor cells after spinal cord injury. J Neurosci Res. 65 (6): 500-7. Summary: The reaction of oligodendrocyte progenitor cells (OPCs) after spinal cord injury (SCI) is poorly understood. In this study, we examined oligodendroglial reactions after contusion SCI in adult rats by immunohistochemistry. OPCs were identified by staining with monoclonal antibodies (mAbs) A2B5 and O4. Each of the A2B5-, O4-positive OPCs and galactocerebroside-positive oligodendrocytes dramatically increased in the lesion of the dorsal posterior funiculus. Bromodeoxyuridine (BrdU) incorporation studies showed that most O4-positive cells in the lesion were labeled with BrdU, suggesting that these OPCs were proliferative. In contrast, the expression of myelin basic protein was decreased in the lesion compared with controls that received laminectomy only. From the injured cord, OPCs were isolated by immunopanning with mAb A2B5. We observed an increased number of OPCs from the injured spinal cords compared with those isolated from controls and unoperated animals. After several days in culture, the OPCs from the lesion expressed galactocerebroside. These results suggest that OPCs are induced and can differentiate following SCI in the adult rat. Copyright 2001 Wiley- Liss, Inc. <http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?db=m&form=6&dopt=r&uid=11550218> Department of Orthopaedic Surgery, Keio University School of Medicine, Shinjuku-ku, Tokyo. [This message was edited by Wise Young on September 23, 2001 at 10:13 PM.]

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09-21-2001, 09:57 PM	#2
Wise Young Administrator Join Date: Jul 2001 Location: New Brunswick, NJ, USA Posts: 34,097	This paper confirms what many scientists have long speculated about. Injury causes a stem cells to produce oligodendroglial precursor cells (OPCs) that then produce oligodendroglial cells that remyelinate the spinal cord. This is true of the rat and presumably also true of humans. Wise.