Mesenchymal Stem Cell Graft Improves Recovery after SCI

wildwilly · 07-06-2012, 05:20 PM

PLoS One. 2012;7(6):e39500. Epub 2012 Jun 20.

Mesenchymal Stem Cell Graft Improves Recovery after Spinal Cord Injury in Adult Rats through Neurotrophic and Pro-Angiogenic Actions.

Quertainmont R, Cantinieaux D, Botman O, Sid S, Schoenen J, Franzen R.
Source

GIGA Neurosciences, Axonal Regeneration and Cephalic Pain Unit, University of Liege, Liege, Belgium.
Abstract

Numerous strategies have been managed to improve functional recovery after spinal cord injury (SCI) but an optimal strategy doesn't exist yet. Actually, it is the complexity of the injured spinal cord pathophysiology that begets the multifactorial approaches assessed to favour tissue protection, axonal regrowth and functional recovery. In this context, it appears that mesenchymal stem cells (MSCs) could take an interesting part. The aim of this study is to graft MSCs after a spinal cord compression injury in adult rat to assess their effect on functional recovery and to highlight their mechanisms of action. We found that in intravenously grafted animals, MSCs induce, as early as 1 week after the graft, an improvement of their open field and grid navigation scores compared to control animals. At the histological analysis of their dissected spinal cord, no MSCs were found within the host despite their BrdU labelling performed before the graft, whatever the delay observed: 7, 14 or 21 days. However, a cytokine array performed on spinal cord extracts 3 days after MSC graft reveals a significant increase of NGF expression in the injured tissue. Also, a significant tissue sparing effect of MSC graft was observed. Finally, we also show that MSCs promote vascularisation, as the density of blood vessels within the lesioned area was higher in grafted rats. In conclusion, we bring here some new evidences that MSCs most likely act throughout their secretions and not via their own integration/differentiation within the host tissue.

http://www.ncbi.nlm.nih.gov/pubmed/22745769

julieshrive · 07-29-2012, 08:26 AM

NB London UK based

re: Chronic utis & bacteriall vaginitus/ trichomunus [ metronizadole? ]

Research in past[ Prof Moorhead kidneys & Brumfitt [ urology] Royal Free
showed spina bifida GPs say occulta but results 1969 didn't mention what
kind.My mobility lifting & carrying poor but suspect connected to breathing
/ heart [ cardiac arrest] due to not following up.

Cupping [ acupuncture] caused a bruise on sacrum [ where spina bifida is]
but GPs are looking me in the face saying osteo /spasm giving medication
that has no effect.Also have CFS / fibro .

I am having the acute problem of episodes of temporary acute pain & near
paralysis caused by chronic utis 3 weekly [ out of Profs control [ not his
remit] relieved with paramol ,acupuncture & adding to uti medication which
can stop within hours but taking longer each time.Now can't get paramol.

The last time I had acute episodes of symptons[ different] resulted in
cardiac arrest as a result never having seen a cardiologist.There is a
possible genetic multi infart dementia connection [ Mother has severely]

What I need patient orientated researchers. Any trials? Stem cells to stop
infections getting to spine?

Maybe I could be the first human as pain excrutiating I don't want it to become chronic with the paralysis .

07-06-2012, 05:20 PM	#1
wildwilly Moderator Join Date: Sep 2004 Location: Michigan Posts: 1,780	Mesenchymal Stem Cell Graft Improves Recovery after SCI PLoS One. 2012;7(6):e39500. Epub 2012 Jun 20. Mesenchymal Stem Cell Graft Improves Recovery after Spinal Cord Injury in Adult Rats through Neurotrophic and Pro-Angiogenic Actions. Quertainmont R, Cantinieaux D, Botman O, Sid S, Schoenen J, Franzen R. Source GIGA Neurosciences, Axonal Regeneration and Cephalic Pain Unit, University of Liege, Liege, Belgium. Abstract Numerous strategies have been managed to improve functional recovery after spinal cord injury (SCI) but an optimal strategy doesn't exist yet. Actually, it is the complexity of the injured spinal cord pathophysiology that begets the multifactorial approaches assessed to favour tissue protection, axonal regrowth and functional recovery. In this context, it appears that mesenchymal stem cells (MSCs) could take an interesting part. The aim of this study is to graft MSCs after a spinal cord compression injury in adult rat to assess their effect on functional recovery and to highlight their mechanisms of action. We found that in intravenously grafted animals, MSCs induce, as early as 1 week after the graft, an improvement of their open field and grid navigation scores compared to control animals. At the histological analysis of their dissected spinal cord, no MSCs were found within the host despite their BrdU labelling performed before the graft, whatever the delay observed: 7, 14 or 21 days. However, a cytokine array performed on spinal cord extracts 3 days after MSC graft reveals a significant increase of NGF expression in the injured tissue. Also, a significant tissue sparing effect of MSC graft was observed. Finally, we also show that MSCs promote vascularisation, as the density of blood vessels within the lesioned area was higher in grafted rats. In conclusion, we bring here some new evidences that MSCs most likely act throughout their secretions and not via their own integration/differentiation within the host tissue. http://www.ncbi.nlm.nih.gov/pubmed/22745769 __________________ “As the cast of villains in SCI is vast and collaborative, so too must be the chorus of hero's that rise to meet them” Ramer et al 2005

Thread Tools
Show Printable Version Email this Page
Display Modes
Linear Mode Switch to Hybrid Mode Switch to Threaded Mode

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
Mesenchymal Stem Cell Graft Improves Recovery after Spinal Cord Injury in Adult Rats	ineedmyelin	Cure	0	07-03-2012 06:24 PM
Inhibition of Apoptotic Cell Death by Ghrelin Improves Functional Recovery	wildwilly	SCI (Related) Research	0	06-26-2010 06:47 AM
Mesenchymal Stem Cells reduces demyelination and induces clinical recovery	wildwilly	Stem Cell Research	0	03-31-2009 10:25 AM
Mesenchymal stem cells elicit function recovery after SCI in rhesus monkeys	Steven Edwards	Stem Cell Research	0	06-24-2006 08:53 PM
Lee, et al. (2003). Minocycline reduces cell death and improves functional recovery after ... SCI	Wise Young	SCI (Animal) Research	0	11-04-2003 02:26 PM

07-29-2012, 08:26 AM	#2
julieshrive Junior Member Join Date: Jul 2012 Posts: 5	NB London UK based re: Chronic utis & bacteriall vaginitus/ trichomunus [ metronizadole? ] Research in past[ Prof Moorhead kidneys & Brumfitt [ urology] Royal Free showed spina bifida GPs say occulta but results 1969 didn't mention what kind.My mobility lifting & carrying poor but suspect connected to breathing / heart [ cardiac arrest] due to not following up. Cupping [ acupuncture] caused a bruise on sacrum [ where spina bifida is] but GPs are looking me in the face saying osteo /spasm giving medication that has no effect.Also have CFS / fibro . I am having the acute problem of episodes of temporary acute pain & near paralysis caused by chronic utis 3 weekly [ out of Profs control [ not his remit] relieved with paramol ,acupuncture & adding to uti medication which can stop within hours but taking longer each time.Now can't get paramol. The last time I had acute episodes of symptons[ different] resulted in cardiac arrest as a result never having seen a cardiologist.There is a possible genetic multi infart dementia connection [ Mother has severely] What I need patient orientated researchers. Any trials? Stem cells to stop infections getting to spine? Maybe I could be the first human as pain excrutiating I don't want it to become chronic with the paralysis .