Research article
Ascending central canal dilation and progressive ependymal disruption in a contusion model of rodent chronic spinal cord injury
Milan Radojicic , Gabriel Nistor and Hans S Keirstead

BMC Neurology 2007, 7:30 doi:10.1186/1471-2377-7-30

Published 7 September 2007


Chronic spinal cord injury (SCI) can lead to an insidious decline in motor and sensory function in individuals even years after the initial injury and is accompanied by a slow and progressive cytoarchitectural destruction. At present, no pathological mechanisms satisfactorily explain the ongoing degeneration.


Adult female Sprague-Dawley rats were anesthetized laminectomized at T10 and received spinal cord contusion injuries with a force of 250 kilodynes using an Infinite Horizon Impactor. Animals were randomly distributed into 5 groups and killed 1 (n=4), 28 (n=4), 120 (n=4), 450 (n=5), or 540 (n=5) days after injury. Morphometric and immunohistochemical studies were then performed on 1 mm block sections, 6 mm cranial and 6 mm caudal to the lesion epicenter. The SPSS 11.5 t test was used to determine differences between quantitative measures.