Research article
.
Ascending central canal dilation and progressive ependymal disruption in a contusion model of rodent chronic spinal cord injury
Milan Radojicic , Gabriel Nistor and Hans S Keirstead

BMC Neurology 2007, 7:30 doi:10.1186/1471-2377-7-30

Published 7 September 2007

Background

Chronic spinal cord injury (SCI) can lead to an insidious decline in motor and sensory function in individuals even years after the initial injury and is accompanied by a slow and progressive cytoarchitectural destruction. At present, no pathological mechanisms satisfactorily explain the ongoing degeneration.

Methods

Adult female Sprague-Dawley rats were anesthetized laminectomized at T10 and received spinal cord contusion injuries with a force of 250 kilodynes using an Infinite Horizon Impactor. Animals were randomly distributed into 5 groups and killed 1 (n=4), 28 (n=4), 120 (n=4), 450 (n=5), or 540 (n=5) days after injury. Morphometric and immunohistochemical studies were then performed on 1 mm block sections, 6 mm cranial and 6 mm caudal to the lesion epicenter. The SPSS 11.5 t test was used to determine differences between quantitative measures.

more:

http://www.biomedcentral.com/1471-2377/7/30/abstract