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Thread: Kiel, et al. (2005). SLAM family receptors distinguish hematopoietic stem and progenitor cells and reveal endothelial niches for stem cells.

  1. #1

    Kiel, et al. (2005). SLAM family receptors distinguish hematopoietic stem and progenitor cells and reveal endothelial niches for stem cells.

    This is a very important paper from the Morrison laboratory at the University of Michigan. Many researchers have been looking for markers of hematopoietic stem cells and this is the first credible demonstration that SLAM family of receptors (CD150, CD244, and CD48) allow the separation of hemapoietic stem cells (CD150 only), multipotent hematopoietic stem cells (CD244+ only), and restricted hematopoietic progenitors (CD48+ and CD244+). With these markers, they were able to visualize where the hematopoietic stem cells are present in bone marrow and they foudn that the cells were present in a number of "niches" including sinusoidal endothelium in spleen and bone marrow.

    Kiel MJ, Yilmaz OH, Iwashita T, Terhorst C and Morrison SJ (2005). SLAM family receptors distinguish hematopoietic stem and progenitor cells and reveal endothelial niches for stem cells. Cell 121: 1109-21. To improve our ability to identify hematopoietic stem cells (HSCs) and their localization in vivo, we compared the gene expression profiles of highly purified HSCs and non-self-renewing multipotent hematopoietic progenitors (MPPs). Cell surface receptors of the SLAM family, including CD150, CD244, and CD48, were differentially expressed among functionally distinct progenitors. HSCs were highly purified as CD150(+)CD244(-)CD48(-) cells while MPPs were CD244(+)CD150(-)CD48(-) and most restricted progenitors were CD48(+)CD244(+)CD150(-). The primitiveness of hematopoietic progenitors could thus be predicted based on the combination of SLAM family members they expressed. This is the first family of receptors whose combinatorial expression precisely distinguishes stem and progenitor cells. The ability to purify HSCs based on a simple combination of SLAM receptors allowed us to identify HSCs in tissue sections. Many HSCs were associated with sinusoidal endothelium in spleen and bone marrow, though some HSCs were associated with endosteum. HSCs thus occupy multiple niches, including sinusoidal endothelium in diverse tissues. Howard Hughes Medical Institute and Department of Internal Medicine, University of Michigan, Ann Arbor, Michigan 48109, USA. http://www.ncbi.nlm.nih.gov/entrez/q..._uids=15989959

  2. #2
    Commentary on the work by Morrison.

    Wagers AJ (2005). Stem cell grand SLAM. Cell 121: 967-70. Stem cells in both embryonic and adult tissues are defined by their unique ability to balance self-renewal and differentiation such that mature cells necessary for tissue function can be generated and replaced without depletion of the stem cell pool. In this issue of Cell, report a major step forward for studying the mechanisms and regulation of such stem cell fate decisions in the blood-forming (hematopoietic) system by providing a simple and broadly applicable method to isolate these cells and to visualize them in their normal environment. Joslin Diabetes Center, Boston, Massachusetts 02215, USA. http://www.ncbi.nlm.nih.gov/entrez/q..._uids=15989946

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