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Thread: Hasegawa, et al. (2005). Embryonic radial glia bridge spinal cord lesions and promote functional recovery following spinal cord injury.

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    Hasegawa, et al. (2005). Embryonic radial glia bridge spinal cord lesions and promote functional recovery following spinal cord injury.

    This study from the Keck Center at Rutgers reports that embryonic radial glial cells (these are neural stem cells that have been "immortalized" with v-myc) migrate extensively when transplanted into contused rat spinal cords and promotes axonal regeneration and functional recovery. They compared non-immortalized and immortalized neural stem cells, finding that the latter stimulate more growth and functional recovery than the former. This suggests that when neural stem cells are transplanted into the spinal cord, the rate at which they differentiate into astrocytes and other cells may influence their ability to stimulate and support regeneration.

    Hasegawa K, Chang YW, Li H, Berlin Y, Ikeda O, Kane-Goldsmith N and Grumet M (2005). Embryonic radial glia bridge spinal cord lesions and promote functional recovery following spinal cord injury. Exp Neurol 193: 394-410. Radial glial cells are neural stem cells (NSC) that are transiently found in the developing CNS. To study radial glia, we isolated clones following immortalization of E13.5 GFP rat neurospheres with v-myc. Clone RG3.6 exhibits polarized morphology and expresses the radial glial markers nestin and brain lipid binding protein. Both NSC and RG3.6 cells migrated extensively in the adult spinal cord. However, RG3.6 cells differentiated into astroglia slower than NSC, suggesting that immortalization can delay differentiation of radial glia. Following spinal cord contusion, implanted RG3.6 cells migrated widely in the contusion site and into spared white matter where they exhibited a highly polarized morphology. When injected immediately after injury, RG3.6 cells formed cellular bridges surrounding spinal cord lesion sites and extending into spared white matter regions in contrast to GFP fibroblasts that remained in the lesion site. Behavioral analysis indicated higher BBB scores in rats injected with RG3.6 cells than rats injected with fibroblasts or medium as early as 1 week after injury. Spinal cords transplanted with RG3.6 cells or dermal fibroblasts exhibited little accumulation of chondroitin sulfate proteoglycans (CSPG) including NG2 proteoglycans that are known to inhibit axonal growth. Reduced levels of CSPG were accompanied by little accumulation in the injury site of activated macrophages, which are a major source of CSPG. However, increased staining and organization of neurofilaments were found in injured rats transplanted with RG3.6 cells suggesting neuroprotection or regrowth. The combined results indicate that acutely transplanted radial glia can migrate to form bridges across spinal cord lesions in vivo and promote functional recovery following spinal cord injury by protecting against macrophages and secondary damage. W. M. Keck Center for Collaborative Neuroscience, 604 Allison Road, Rutgers, State University of New Jersey, Piscataway, NJ 08854-8082, USA. http://www.ncbi.nlm.nih.gov/entrez/q..._uids=15869942
    Last edited by Wise Young; 08-03-2005 at 07:16 PM.

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