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Thread: Stem cells from umbilical cord blood reduce stroke size and damage.

  1. #1

    Stem cells from umbilical cord blood reduce stroke size and damage.

    http://www.news-medical.net/?id=5057
    Stem cells from umbilical cord blood, given intravenously with mannitol drug reduce stroke size and damage
    Medical Research News
    9/26/04

    Stem cells taken from umbilical cord blood, then given intravenously along with a drug known to temporarily breach the brain's protective barrier, can dramatically reduce stroke size and damage, Medical College of Georgia and University of South Florida researchers say.
    "What we found was interesting, phenomenal really," says Dr. Cesario V. Borlongan, neuroscientist and lead author of the study published in the October issue of the American Heart Association journal, Stroke.

    Researchers first gave the drug, mannitol, to provide temporary passage through the blood-brain barrier then transfused human umbilical cord blood cells into a stroke animal model. When used in the first hours and days following a stroke, stroke size decreased by 40 percent and resulting disability was significantly reduced.

    "We have two potential routes of delivery, intravenously through the jugular vein or directly transplanting the cells into the brain," says Dr. Borlongan. Initial studies comparing the two approaches showed the intravenous approach ineffective until researchers added mannitol.

    "Intravenous delivery of (human umbilical cord blood cells) poses an efficient and non-invasive cell therapy for (central nervous system) disorders characterized by a narrow therapeutic window," the researchers write. "A multi-drug treatment for stroke may be realized via a cell-based therapy that involves routine clinical IV infusion of stem/progenitor cells allowing the biological release of a cocktail of trophic factors into the brain."

    Although no evidence could be found of the low-dose umbilical cord blood stem cells themselves in the brain three days after the treatment, evidence of neuroprotection was clear, Dr. Borlongan says. "The critical factors we have seen elevated in this stroke animal model are the neurotrophic factors."

    "This is an important finding because it shows that umbilical cord blood cells do not have to become new brain cells to protect the brain," says Dr. Paul R. Sanberg, neuroscientist at the University of South Florida and a co-author on the study. Human umbilical cord blood stem cells used for the study were obtained from Saneron CCEL Therapeutics, Inc., in Tampa, Fla., a USF spin-off company researching clinical applications for cord blood cells.

    An article in the same issue of Stroke by the USF researchers led by Dr. Alison Willing, neuroscientist, shows that 10 times the number of stem cells or more would be needed to produce similar results if cells were given intravenously without any help crossing the blood-brain barrier.

    The MCG-led study used 200,000 umbilical cord blood cells and a low dose of mannitol, a sugar alcohol and diuretic whose uses include helping chemotherapeutic agents reach the brain. The USF study showed a dose-response relationship with significant recovery in behavioral performance when 1 million or more cells were given, Drs. Willing and Sanberg and their colleagues report.

    "The advances we are seeing here are with mannitol, you can deliver a smaller number of cells and it can protect against stroke," Dr. Borlongan says. "It can reduce the size of infarction; it can lead to behavioral improvement," an important implication for clinical applications because stem cells are difficult to harvest.

    "By using mannitol or similar drugs, it may be possible to treat stroke patients in the future with cells from only a few umbilical cords," adds Dr. Sanberg.

    The Stroke paper also explored how stem cells provide neuroprotection. The researchers speculated it was by providing the large influx of nourishing neurotrophic factors secreted by the stem cells. To test that theory, they looked at what happened when they used antibodies that negated some of the factors. "When we blocked the neurotrophic factors, it blocked the positive effect," Dr. Borlongan says.

    The study also looked at mannitol as a therapy because of previous reports it reduces cerebral swelling and stroke damage. Researchers found that at the same low dose used to accompany the stem cells, mannitol alone was ineffective. "The dosage we are using is probably 10 times lower than the one showing mannitol itself to be protective," Dr. Borlongan says.

    But those higher doses come with higher risks as tight cell junctions that prevent many molecules from reaching the brain relax for longer periods, leaving the brain susceptible to problems such as infection.

    Drs. Borlongan and Willing are consultants and Dr. Sanberg is cofounder of Saneron CCEL Therapeutics, Inc.

  2. #2
    I had a treatment of Cord Blood Stem Cell from Biomark and I read a article about Mannitol along with Cord Blood, I showed it to Biomark and I was approved to have a low dose of Mannitol before my stem cell injection. The inject was given intravaneously. After the treat i found it a little easier to walk but nothing really big.
    If anyone wants to know Biomark sold to Advanced Cell Therapeutics http://www.stem-cells.com

    They also treated someone else with a SCI and here is what they emailed me:

    "My son Matthew had the stem cells on 30 March 2005. He is a C6/C7 incomplete. His injury was at that stage 6 months old. He had the Mannitol drip beforehand - it took about 30 minutes to administer an we didn't notice anything at all. 50% of the stem cells were then injected into the drip line and the balance were injected under his skin just above the lesion. This injection was no more uncomfortable than you would expect a prick in the neck to be.
    I would strongly recommend the stem cell injections to anybody with SCI. I regard them as the biggest "cheat" in a successful recovery. Subsequent to the therapy Matt has had phenomenal improvements including his left hand now moving and improving; his sensation returning up to bum level; his muscles in his stomach, hips, bum and upper legs are now working (still weak, but working). With the aid of special ankle boots that allow him to lock his knees, he is able to walk in his parallel bars, with the aid of a physiotherapist (she moves his lower leg, he moves his hips and unlocks his knees). Today he made another big step forward - he pulled himself from seated to legs fully extended, over a big gym ball, by himself - using his leg muscles to get up.

    We have noted improvements and returns from 3 days afterwards until today. The recovery seemed to be such that we could note it every week and now we can see things improving daily in his walking, standing and leg ability.

    His overall body strenght is markedly improved, making all exercises and general living that much easier. His eyes improved (couldn't focus properly in 3D after the accident) and his teenaged skin has cleared up. His mood is consistently that much more positive - this may be due to the fact that he can now see the result of his work and is encouraged by it.

    Matt also does about 4 hours intensive therapy per day. I think it is important to know that the stem cell therapy itself HAS to be backed up by lots of work both before and afterwards so that the body gets an idea of what it is supposed to be doing. The doctors call this "signalling". They have to relearn the programme of the nerves, and the best way to do this is show them what you want the body to do. If you are interested I will give you more details on the therapies that have worked for Matt.

    So, do I recommend stem cells for SCI ?- DEFINITELY!!!!!

    Life without stem cells after an injury of this nature is just too depressing to contemplate, as the rate of recovery is so slow. By the way, I have seen two people with complete lumbar lesions who have taught themselves to walk ater about 7 years. I am a firm believer that recovery is possible no matter what, but the stem cells are a quick way of doing it. Let me know what you think.

    Hope this helps.

    Regards,
    Bridget.

    This person had some good results but was only six months post. Who knows what would of happened w/out the stem cells.

  3. #3
    Senior Member Leo's Avatar
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    Rich, thanks for sharing this, i have a friend with MS who's thinking of trying this. Your right so much can happen after 6 months of SCI.
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