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Thread: A. S. Bruhovetsky's fetal stem cell treatment of chronic spinal cord injury in Moscow

  1. #1

    A. S. Bruhovetsky's fetal stem cell treatment of chronic spinal cord injury in Moscow

    Professor A. S. Burhovetsky has transplanted fetal stem cells into about 15 patients with chronic spinal cord injury. Here is a brief summary of the procedure as the doctor describes in an email to the father of person with spinal cord injury (I edited the text for grammar and clarity but tried to leave the message in as much of the original words as possible):

    We have treated about 15 patients with SCI. The results depend on degree of damage of spinal cord. We got improvement in movement function in 5 cases, significant recovering sensitivity in 8 cases, eliminating spasticity in 9 cases, restoration of bowel and bladder function in 7 cases. Every case has peculiarity. We develop individual transplant strategy for every patient.

    There are two approach of treatment:

    1. Low invasive method. It includes diagnostic spinal angiography, immunosupression, then regional program perfusion for the purposes of pharmacological preparation of the damaged brain for cellular transplantation, subarachnoid (suboccipital, lumbar) transfusion , embryonal stem neuronal cell transplantation, and transcranial magnetic stimulation.

    2. Large neurosurgical operation
    • Interlaminectomy(or laminectomy) at the injury level.
    • Opening dura muter on injury level.
    • Radiculomyelolis.
    • In case of presence of myelocysts at this level - emptying them .
    • Intraoperational neuromyografical analysis of spinal cord conduction .
    • Intramedular Schwann/OGE/neuroglial embryonic cell transplantation.
    • Plasty of a solid cerebral membranes. (dura mater)
    • Transcranial magnetic stimulation

    The cost of treatment depends on your treatment program and conditions of your stay in hospital ( it means what kind of room you suit). So the costs (for treatment) vary from $9000 to $50000. A single room costs about $50 per day, double room - about $150, and VIP suit -about 350 $ per day

    In order to decide, if your son can be accounted a candidate for our treatment we ask you to carry out padding researches and to send them to our address:
    • MRI (3 Months. or Less) , if its impossible we ask you to send the result of CAT Scan (3 Months. or Less ) , made for damage zone.
    • Original X-Ray of spine, performed in 2 projection.
    • Analysis of blood serum on neurospecific enolase , glial fibrilar acid protein and antibody to it.
    • Your Medical Report
    • Neuromyographical analysis of spinal cord conduction

    Unfortunately, we haven't published in any foreign magazines.

    Professor Bruhovetsky

    Contact Information:
    Prof. Bruhovetskiy Andrei Stepanovitch,
    tel. Kupavna:527 80 10 post.-443.
    assistant-190 72 92-Svetlana

    [This message was edited by Wise Young on August 04, 2001 at 08:56 AM.]

  2. #2

    Additional Information about the Institute that Professor Bryukhovetsky is located

    The laboratory for cell transplantation surgery and bioengineering (hereinafter referred to as "the Laboratory") has been established on June 5th, 2000 within the scientific- research institute of transplantation surgery and artificial organs of the Ministry of Health of the Russian Federation under the permission issued by the Ministry of Health of the Russian Federation (No.25101513826 dated 06.05.2000). The founders of the Laboratory are as follows: the scientific- research institute of transplantation surgery and artificial organs of the Ministry of Health of the Russian Federation (Russia, Moscow) and the financial group "Sovereign" (Forex) (Switzerland, Zurich).

    The purpose: Development in course of experiments and introduction into clinical practice high medical technologies of cell transplantation surgery and bioengineering methods in case of diseases and traumas of brain and spinal marrow.

    The Laboratory operates within the framework of international scientific-research program "Cell transplantation surgery and bioengineering of human organs" (see Appendix 1). Scientific leader of the program, head of the high-tech group of the scientific-research institute of transplantation surgery and artificial organs of the Ministry of Health of the Russian Federation, professor of the chair of biomedical instruments and technologies of MGAPI, Doctor of Medical Science, Bryukhovetsky Andrey Stepanovich.

    The Laboratory consists of experimental and clinical departments, and it also has its culture boxes and cell banks. Bioengineering work is performed by specialists of the Laboratory in the leading medical institutions of Russia.

    Clinical bases:

    1. The scientific-research institute of transplantation surgery and artificial organs of the Ministry of Health of the Russian Federation (Moscow).

    2. Central clinical bath hospital (Moscow)

    3. Central military clinical hospital named after A.V.Vishnevsky (the town of Arkhangelskoye, the Moscow Region).

    4. Central navy clinical hospital (the town of Kupavna, the Moscow Region).

    Experimental-laboratory bases:

    1. State scientific centre for social and forensic psychiatry named after V.P.Serbsky (Moscow).

    2. Russian university of peoples' friendship (Moscow).

    3. The scientific-research institute of transplantation surgery and artificial organs of the Ministry of Health of the Russian Federation (Moscow).

    The experimental materials are given in the Laboratory's reports (see "reports").

    The reports on patient healing by this method were submitted to the Ministry of Health of the Russian Federation in 1999.

    The materials for clinical cell transplantation are being prepared in State scientific centre for social and forensic psychiatry named after V.P.Serbsky on the basis of resolution issued by the Authority of state control of medicines and medical instruments of the Ministry of Health of the Russian Federation, No.29-3/826 dated October 7, 1997.

    Healing the patients by this method has been performed in the scientific-research institute of transplantation surgery and artificial organs of the Ministry of Health of the Russian Federation since 1995 within the framework of inter- departmental program "Neurotransplantation and reconstruction- restoration transplantation in diseases and traumas of central nervous system (CNS) and locomotor apparatus". The methods are patented in the Russian Federation as a method for bioengineering brain plasty (3 patents of Russia, No.2146932 dated Nov.12, 1998; No.2152038 dated Nov.26, 1998, and No.2152038 dated Dec.9, 1998 were issued). The work results were heard at the meetings of the Scientific board of the scientific-research institute of transplantation surgery and artificial organs of the Ministry of Health of the Russian Federation in 1997, 1999 and 2000.

    Methods for healing

    Stages of bioengineering brain plasty:

    Preparatory stage consists of the two parts:

    Part 1: Preparation of materials for bioengineering

    a) Collection, culture breeding and standardisation of brain bubble tissue of a human embryo for 7-12 weeks of gestation; tissue dissociation into separate cells; cell dividing in culture by types, and separate cultivation; assessment of viability; preparation of specialised cell transplant (neuronal, neuronal-glial or neuroglial in different scripts and quantities) compatible with the recipient, as far as the blood group and Rhesus factor are concerned (a scheme of cell transplant preparation is presented).

    b) information assessment of compatibility of the indices of electromagnetic field of biological liquids of the patients (blood, urine, liquor) and donor's cell transplant; if necessary, the transplant material is undergone to electromagnetic irradiation within mm range, acoustic- electromagnetic waves, bioresonance effects. A method (patented in Russia) by the Moscow institute of information wave technologies (Director professor, Doctor of engineering Khokkanen, M.V.) (a diagram of wave parameter evaluation is presented) is used

    c) computed tomography of brain with densitometric analysis of the intra-tissue reconstruction area; calculation of the quantity of necessary cell material (photographs of densitometric analysis of brain tomography) is performed.

    d) study of neurospecific blood proteins and antibodies to them is performed, and conclusion is drawn on the possibility of cell transplantation of nervous tissue to this patient.

    Part 2: Preparation of the patient to operation

    a) preparation of an access area for the operation: under the conditions of radiography-equipped operation room in pathologic area of the brain, in which bioengineering brain plasty is to be performed, vascular network is studies by angiography, and at the end, a permanent artery cannula for the program regional perfussion of medicines is fixed, and then catheters are fixed or preparation of all the necessary instruments and medicines for puncture intravascular or surgical introduction of bio- material and biologically active substances is performed (see photograph of polyvector effect).

    b) standard pre-operation preparation of the patient; preliminary drug administering; and the patient is moved to neuroreanimation department, in which subclavian vein cannula is fixed, and the patient is connected to cardio- and encephalographic monitoring equipment (see photograph of neuroreanimation).

    Stage 1: Re-modelling of mainstream and microcircullar bed

    After the review cerebral or spinal angiography by the use of the cardiovascular complex, Intergis 3000 (Philips) (see photograph), or angiographic complex (Siemens), the analysis of the existing haemodynamics is carried out and anatomy of mainstream and microcircullar vascular bed is studied; if a pathology is found, intra-arterial regional perfusion of a system of antireagents, vasoactive substances and preparations to improve local blood microcirulation and rheology, as well as the vascular growth factor is performed into the pathologic brain area, which allows to create or restore regional microcirculation network in the brain area through the formation of collateral blood circulation during all the stages of the operation (photographs of four angiographs of the restoration of reserve brain vessels after the treatment).

    Efficiency analysis of this stage is made by the use of computer-assisted method of angiographic image linearisation (see colour insert of angiogramms).

    At this stage metabolic support and protection of the brain is carried out through intra-arterial infusion into mainstream arteries of the head with the solution of nucleic acids and specific tissue proteins, which results in activation of genetic apparatus of the cells in pathologic area and enhancement of brain sanogenesis reserve.

    Stage 2: Neuro-transplantation

    Transplantation or citotransfusion of embryo cells of nerve tissue into the sub-menix area of the brain to create biologically active substances in a focus of pathology and co- operation of cell metabolism under the background of tissue- stimulating artery perfusion with biologically active substances. Extended retardation of exchange processes in the brain with general system medicines is also performed by:

    a) central controlled anaesthesia, or

    b) surfacial projective craniocerebral laying with ice bags or the use of hypothermal technique.

    Purpose-oriented cell intervention is carried out by:

    1. Direct transplantation of stem nerve cells into a certain brain area (see photograph) in neurosurgrical operations.

    2. Citotransfusion into subarachnoidal area of the brain (endolumbar or suboccipital introduction of stem nerve cells) (see photograph).

    3. Citotransfusion of stem nerve cells into the ventricles of the brain (see photograph).

    4. Stereotaxic transplantation of stem nerve cells (see photograph).

    5. Microndoscopic transplantation of stem nerve cells (see photograph).

    6. Supporting tissue program therapy by the transplantation of cells under aponeurosis of direct abdominal muscles.

    At this stage, possible post-transplantation damages of vital functions and complications are corrected by standard methods under the conditions of the department of neuroreanimation and intensive therapy; if necessary, arterial, subarachnoidal introduction of antiobiotics, symptomatic therapy, etc are performed.

    Stage 3: Activation and protection of transplant

    Infusion of factors stimulating different sides of transplant development (factors of nerve cell growth, factors of astrocitar or oligodenroglial activation or enhancement of synaptic transfer, etc) is performed through the damaged GEB regionally. In addition, perfusion of medicinal and biologically active substances providing the closure of haematoencephalic barrier is performed, metabolic residues are removed by the standard endotoxic methods (hemosorbtion, lymphosorbtion, plasmoferesis, liquoferosis, etc). Method of blocking the astrocitar and back-cervical sympathetic nodes for denervation of vegetative supply to brain in the transplantation area. The KHF therapy is used on large joints for the integration of somatic and vegetative components of the brain plasty area

    Final stage

    Massive temporal or long regional artery perfusion of: antihypoxants, ozone, blood preparations, plasma, etc, control angiography to avoid regional thrombosis, removal of artery cannula, active rehabilitation and training of former brain functions, restoration of damaged brain functions caused as a result of treatment or their active stimulation by exercise therapy, massage, electrostimulation, KHF irradiation, etc.

    To restore motor functions a program of specialised exercise therapy is performed in the centre of kinesetherapy by S.M.Bubnovsky's method.

    It should be noted that contemporary fundamental researches on wave genetic code (Garyayev, P.P., 1997; Berezin, A.A., 1998), achievements of endovascular roentgenosurgery on long-term regional perfusion of medicines (Selivanov, E.A., Khanevich, M.D., 1997, 1998; Saveliev, V.S., 1986; Zubritsky, V.F., Bryukhovetsky, A.S., 1998).) and cellular transplantology (Shumakov, V.I., Skaletsky, A.E., 1997; Sukhikh, G.T., Molnar, E., 1995; Gaidar, B.V. et al, 1996, Bryukhovetsky, A.S., 1998) were a basis of this technology, and these works are the theoretical background to the proposed method of treatment of severe organic diseases and traumas of the brain. As a result of the said works it was proved that the somatic tissue cells had high plasticity (Kotlyar, B.I., 1986), they are able to change morphologic structure of nuclei's DNA of their cells followed by acoustic-electromagnetic irradiation (Garyayev, P.P., 1997) and irradiation in mm range (Deviatkov, Golant, Beletsky, 1996), provide co-operation of cell methabolism, activation and transformation of genetic resources of cells to restore the function of the organ. The bio-engineering approach is based on 10-year fundamental experimental and practical researches at the laboratory of immunology in the State scientific centre for social and forensic psychiatry named after V.P.Serbsky on the influence of neurospecific human brain proteins on separate structural elements of nerve, cerebrovascular tissue and GEB (Chekhonin, V.P. et al, 1996, 1997, 1998). It was proved in theory and practice that it is possible to replace pathological cell elements of the brain for embryonal donor cells with their direct transvascular citotransfusion into the brain, as well as modulation of functional condition of the organ in transplantation of cells into its submeninx area (Bryukhovetsky, A.S., 1997, 1998). However each of these methods does not allow to reach stable clinical effect, neither in theory nor in practice, which is possible only in case of program application of these methods within the framework of highly specialised bio-engineering algorithm.

  3. #3

    More about the Russian transplant program

    International scientific research program

    Â*<>?---?€? "??-?? <> / Select area --------------- Frequently Asked Questions If you have any questions? How to find us --------------- Our services Official and legal fields Official and legal fields Methods Operation stages Program of treatment Clinics Indication to treatment Patient card --------------- About program Program passport Program leaders Program description Patents Reviews Contacts between the doctors

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    Program description

    1. Content of Problem and Justification of Solving Hereof by Programmable Facilities

    The International Research Program "Cell Transplantation Surgery and Tissue Engineering for Brain Diseases and Brain Injury" (hereinafter called Program) has been developed in compliance with the Order of the Chief Transplantation Surgeon of the Russian Federation - Director of Transplantation Surgery and Artificial Organs Research Institute of March 14, 2000 pursuant to the appeal of the Head of Financial Group "Sovereign" (Switzerland) of 13.03.2000 and Private Joint-Stock Company "Fakel" (Russia) of 01.03.2000, as well as in conjunction with approval of joint scientific research with the participation of the neuromorphological laboratory of the State University of the city of Zurich (Switzerland) and establishment of Joint Russian-Swiss Enterprise "Cell Transplantation and Body Organs Bioengineering Laboratory" with the aim to ensure application in medicine practice of up-to-date achievements in the field of cell transplantation and clinical bioengineering.

    Application of biotechnologies in medicine opens great possibilities for creating known as well as new forms of substance (E.Drexler, 1995, 1999). Analytical reviews of specialized reference documents on technical and social consequencies of development of bioengineering technologies in the field of biology and public health services prognosticate even today a possibility of conducting analysis and reconstructing cell structures responsible for bearing the genetic code, actual mechanisms of controlling biochemical processes in upper-level tissues, stimulating formation of healthy cells instead of old cells. This type of control opens up unique possibilities of biosynthesis of various parts of human body, artificial organs and various versions of creating new organisms from cloning to synthesis according to a predetermined program, that is not possible to obtain today in traditional medico-biological processes. Initial distrust to these prognostications gives place to concrete facts and actual achievements obtained in this field. Main biotechnological results of today are expressed in the realization of the following processes: field desorption and stimulated chemical etch, precipitation of compounds from gas phase, overcoming of the problem of tyranny of interjunctions and thermal explosion, realization of cellular bioassembly and tissue reconstruction. A series of conferences and symposiums on biotechnologies in the USA, Russia, Japan, China and Germany testify of the approach of their practical realization.

    Socio-economic and national economic value of the realization of these problems on the basis of the introduction of bioengineering technologies into clinical medicine is not only of a scientific significance, but is of great national importance. This scientific research should always be supervised by the State. Theoretically, only in the field of biology and public health care such research work on biological and bioengineering issues will make it possible to create operational systems of medical diagnosis that will allow to determine the state of the organism and the type of disease following the reactions of biological sensors in human brain. Development of biosynthesizers on the basis of atom and molecule assemblers will make it possible to synthesize various artificial organs, to breed new varieties of plants, various species of transgenic animals, to synthesize high-quality foods without using the traditional technological cycle. On the basis of bioengineering technologies, it will be possible to create various medico-biological actuators ranging from nanosize to big size and using traditional methods of movement as well as new methods (on the basis of silicon micromechanics, atom transport, etc.). Such technologies will make it possible to accomplish the functions of transport, assembly and disassembly of substance. Employment of molecule synthesizers in pharmacology and biochemistry will create directly required drugs and medicinal preparations, as well as artificial biomaterials with improved features that will allow to increase their service life and decrease their consumption. Besides, total decrease of materials actually in every branch of industry and sphere of the state will allow to abstain from building giants of chemical industry and replace them with mass employment of atom assemblers.

    The specialized expert analysis of biotechnological approaches has proved that possibilities of employment of bioengineering technologies are practically unlimited in all spheres of development of the society. Inevitability of development of bioengineering technologies in the world and scientific, technical and social consequences thereof will actually help the society which uses these results to pass over to a new level of development.

    This task may be solved only by integrating the latest achievements in various fields of science and technology within the framework of developing a new organizational concept. Thus, the achievements of modern transplantation surgery testify of a possibility of radical treatment of a series of diseases which have been incurable till now. Nevertheless, transplantation of organs, tissues and cells traditionally has been developed mainly in the somatic area (transplantation of liver, kidneys, heart, mediastinum organocomplexes, transplantation of cells of pancreas, etc.). On the one hand, the brain has not been practically considered by researchers as subject of transplantation surgery due to its complexity and poor knowledge of its structural and functional organization, absence of technical capabilities for its revival, as well as peculiarities of its regeneration. On the other hand, brain transplantation is actually an insolvable problem not so much due to technical difficulties but mainly due to ethical considerations. Bioengineering plasty of brain tissue may become an alternative to brain transplantation. It will allow reconstruction of separate components and structures of the brain without disrupting the integrity of the brain as the control organ of the most essential systems of the human body, and without disrupting the structure of the person's ego.

    Bioengineering plasty as a new up-to-date medicinal biotechnology has undergone sharp growth during the last five years as one of high technologies of partial transplantation surgery. Transplantation of nerve tissues and cells of mammals has served as a methodological basis of this technology. The basis of its development has been great experimental research work on animals on transplantation of embryonic nerve cells and a revolutionary research work on transplantation of embryonic nerve tissue into human brain. The object of transplantation has not been the brain of an adult donor, but nervous tissue and suspensions of nerve cells of embryos or fetuses of humans and animals. Selection of embryonic nerve tissue for cell transplantations has been explained by the fact that it can revive and establish synaptic contacts with the neurons of the recipient, while a possibility of transplantation of nervous tissues of an adult donor is greatly limited, even in an experiment.

    Well-proven scientific facts of substitution of cells by donor's nerve cells of perished persons or neurogliac components have opened up perspectives of biological design and engineering of disturbed tissue structures. Hereby, the focus of engineering constructions has shifted inside the tissue. The main elements of constructions have become components and structural elements of tissues: neurons, fibroblasts, neuroglia, vegetative and microvessel components, fibers, synapses. This is the main difference between the bioengineering plasty approaches and those of a traditional plastic surgery which operates with integral tissues or sections of tissues. Transplantation of cells of nerve tissues in various combinations has become the first step in clinical cerebral bioengineering technologies.

    The new technology has emerged at the "junction" of up-to-date medical, biological and information technologies that have already become traditional. Clinical bioengineering has appeared as a result of accumulation of a great number of experimental and clinical data, as a cognised necessity of dialectics of clinical thinking.

    One of milestones of development of clinical bioengineering has become introduction into clinical practice of remodelling of arterial vessels by angioplasty techniques and wide use of programmed regional intra-arterial perfusion. Serious fundamental research on restoration of cerebral blood flow, shunting and embolization of brain blood vessels have paved the way to the emergence of this trend. The newly-borne bioengineering of brain tissues has not negated the previously accumulated experience. On the contrary, it has integrated the whole set of accumulated knowledge and experimental results into a new clinical outlook.

    The other milestone of development of clinical bioengineering has become the recognition of achievements and adoption of the idea of necessity to develop cell transplantation in Russia. Cell transplantation has avoided the fate of domestic genetics and cybernetics. Thus, in April 1996 a historic resolution was adopted at the expanded meeting of the Presidium of the Academy of Medical Sciences of the Russian Federation to start developing this scientific trend in Russia. Rapid development of experimental and clinical neurotransplantation surgery abroad, its recognition as a full-fledged scientific discipline by world transplantation surgeons and its inclusion as a separate section in the Schedule of World Congresses of transplantation surgeons (Montreal, 1998), as well as first clinical successes of national neurotransplantation surgery have become to testify of its great perspectives. Today, this research work on cell and tissue transplantation has grown into a separate school and has quite wide clinical use.

    Enthusiasm of separate groups of researchers has given way to fundamental developments in this field. Nowadays, a new national intradepartmental research program has been created and developed in Russia: "Neurotransplantation and Reconstructive-Regenerative Tissue Transplantation in Organic Diseases and Traumas of Nervous System and Locomotor System" (June 1996) approved by a specialized Academic Council of the Transplantation and Artificial Organs Research Institute of the Ministry of Health of the Russian Federation (17.06.96), approved by the Ministry of Health of the Russian Federation, Chief Military Medical Department of the Defense Ministry of the Russian Federation. The program has been compiled in compliance with the Law of the Russian Federation "On Transplantation of Organs and (or) Tissues" of 22.12.92 and in compliance with Order No. 189 of the Minister of Health of the Russian Federation of 10.08.93. In a final form, after long clinical approbation period on a great number of observations, the results of employment of brain bioengineering plasty technology have been reported to and approved by the expanded meeting of the specialized Academic Council of the Transplantation and Artificial Organs Research Institute of the Ministry of Health of the Russian Federation (26.02.99) and presented in the form of a report to the Ministry of Health of the Russian Federation.

    It was the experience of intradepartmental cooperation of working groups that has made it possible to directly approach the problem of clinical neurobioengineering and allowed to develop the concept of treating diseases and traumas of various organs of human body on the basis of principally new methodological positions. A great breakthrough in theoretical and practical bioengineering research has taken place during the last 3-4 years when, under influence of new information and roentgenosurgical medical technologies, cell transplantation has emerged with new clinical content having partially given way to low-invasive surgical technologies: regional programmed perfusion, vessel remodeling, reconstruction of autonomous vegetative nervous system, technologies of bioresonance and acousto-electromagnetic impact, specialized rehabilitation.

    Thus, clinical bioengineering has grown at the junction of various specialities with the methods thereof being the most advanced trends of development of medical and biological sciences.Clinical bio-engineering became a joining subject, an item organising the different fundamental scientific elaboration and achievements of contemporary fundamental science. In addition, its achievements, results and real prospects of growth require the working out of specialised conceptual apparatus of this scientific branch. We have presented a new information methodologic approach to the theory of intra-tissue bio-engineering in the light of modern views and opinions relating to these studies as the methods of biological controlling. We would like to lay emphasis just on this way of bio-engineering growth. We think that common neurosurgery technologies of brain healing will be replaced by information and computer-based technologies as well as information bio-control technologies in the nearest future.

    2. Main objectives and tasks of the Program

    The main objective of the present Program is the elaboration of bio-engineering technology of intra-tissue reconstruction of human organs and creation of scientifically substantiated organisation and technical solutions for the purpose to improve main indices of people health, increase the life expectancy and improve the living standards of the patients suffering from the most severe and hardly curable diseases of the hearth, brain, liver, as well as to provide the working out and introduction into medical practice the modern technologies of cell transplantation surgery.

    Nearest objectives:

    1. To create a basic versatile algorithm for complex application of low-invasion methods of roentgenosurgery, endoviodesurgery, cell transplantation surgery and information-wave effects for distant regional bio-engineering reconstruction of pathologic tissue of the organ (hearth, brain, liver, etc) and for restoration of damaged functions of the organ in the patients and injured.

    2. To work out and introduce the information technologies into the structure of medical institutions with the use of cell transplantation.

    3. To work out recommendations to introduce the developed bioengineering and cell bio-technologies.

    Main tasks:

    1. To work out and introduce into practice a basic algorithm of polyvector effect on focus (of pathology) in the organ (hearth, liver, brain) and the mechanisms of controlling the tissue biosynthesis in the pathologic area in trauma and organic diseases.

    2. To study the possibility of restoration of the damaged blood supply in the pathologic area in diseases of the damaged organ by the use of intra-arterial program regional perfusion of medicines and biologically active preparations as well as to study the brain's reserve capacities of revascularization.

    4. To work out the ways to restore the vegetative innervation of pathologic area in human brain and hearth.

    5. To study the methods of cell bio-assembly and cell transplantation for the purpose to create a new tissue structure in the damaged area of the brain and hearth.

    6. To work out the methods of bioengineering integration of the components of the restored tissue biological structure of the hearth and brain.

    7. To work out the indications and contraindications to the use of bioengineering brain plasty technology, to study possible complications and preventive methods to control them.

    8. To perform mathematical simulation of bioengineering reconstruction of the organ with subsequent optimisation of its structure.

    9. To carry out a system design and development of specialist software and programs (SMPO) for the main stages of biotechnology with subsequent assessment of the efficiency of its introduction.

    10. To work out the recommendations on how to implement the technology in clinical health institutions in the Russian Federation.

    If these tasks are solved, disease incidence and mortality of people in the Russian Federation will be reduced drastically.

    3. Terms and stages for the program realisation

    The program will start from 2000 to 2007, and it will consists of two stages.

    Stage 1 (2000-2001):
    The setting up of Russian-Swiss joint venture "The Laboratory of cell transplantation surgery and human organ bioengineering", the main performer and co-ordinator in the problem; the developing and strengthening of material and technical base of scientific and practical medical institutions of the Program participants; the training of medical and engineering staff; experimental and scientific elaboration of specialist bioengineering technologies of organ tissue reconstruction and their standardisation; the working out of basic measures for information support of the participant institutions.

    Stage 2 (2002-2007):
    Wide introduction of cell transplantation technologies into clinical practice; additional furnishing the leading transplantation centres with necessary facilities; training of highly skilled specialists in the field of clinical bio-engineering of organs and tissues, including the setting up of chairs and courses within the scientific research institute of transplantation surgery and artificial organs of the Ministry of Health of the Russian Federation; wide introduction of low-invasion stereotaxic and microendoscopic medical technologies of cell transplantation surgery.

    4. A system of program measures

    Such a system provides the solution of concrete tasks, that are interrelated and co-ordinated by time, resources and performers, with the account of modern trends of growth of public health, requirements of regulations, factual economic conditions and real experiment results obtained as a result of scientific-research work.

    The program provides stage-by-stage solution of the problem to work out and create bioengineering technologies of tissue reconstruction of human organs on the basis of main achievements of cell transplantation surgery and step-by-step introduction of these technologies into clinical practice. At the same time it is planned to create staff structure, to improve material and technical base, scientific and staff support mainly at the first stage, hiring the most trained and skilled specialists in Moscow so that it can be possible to attain reliable results at the second stage and to introduce them widely into medical practice.

    5. Resources for the Program

    The program is realised at the cost of non-budget financing only. It is provided to use the funds of program customers (private domestic and foreign investors), own funds of Russian-Swiss joint venture "The Laboratory of cell transplantation surgery and human organ bioengineering" gained for scientific-research work and medical services rendering to domestic and foreign patients in the field of cell transplantation surgery. The planned total volume of non-budget financing the Program will be 20,003,900 roubles.

    The figures of financing for the Program are subject to revision on an annual basis in accordance with the established procedure.

    6. Mechanism of the Program realisation

    The Program is performed under the supervision of the Ministry of Health of the Russian Federation on the basis of standard agreements for making the scientific-technical products between head institutions, namely between the scientific research institute of transplantation surgery and artificial organs of the Ministry of Health of the Russian Federation and the Program customers and their representative, joint venture "The Laboratory of cell transplantation surgery and human organ bio-engineering".

    In course of the Program implementing the joint venture "The Laboratory of cell transplantation surgery and human organ bioengineering" co-ordinates the activities of main performers; controls purpose-oriented and effective use of funds and performance of planned measures; makes a summarised order of the Customer together with main performers of the Program; in such order the amounts of funds for subsequent stage-by-stage realisation of the Program measures are to be specified and concrete measures are to be indicated in cost and natural indices; and also presents the report on scientific research work results and its efficiency at each stage. The joint venture "The Laboratory of cell transplantation surgery and human organ bio-engineering" undertakes to provide possible full financing the Program. It organises, checks and provide the execution of measures in full as provided by the Program of work.

    The main performers of the Program shall sign agreements of joint activity with the Head organisation, the scientific research institute of transplantation surgery and artificial organs of the Ministry of Health of the Russian Federation, and agreements for medical service with the joint venture "The Laboratory of cell transplantation surgery and human organ bio-engineering". The joint venture shall settle the Performers' annual plans with the Customer, employ the research teams or hire the most skilled specialists on an employment agreement basis to do concrete scientific researches and work.

    Performers of separate measures of the Program (enterprises, institutions, organisations, public unions and separate specialists) are to be selected on a competition basis in accordance with the Regulations of purchasing the goods, works and services for state needs approved by Decree of the President of the Russian Federation on April 8th, 1997, No.305 "Regarding the urgent measures to prevent corruption and reduce budget expenditures in purchasing the products for state needs".

    In course of the Program realisation the joint venture "The Laboratory of cell transplantation surgery and human organ bio-engineering" will undertake to sign agreements with international organisations to attract additional financing for further development of the Program, strengthening the material and technical base of the performers, and also to develop co-operation and relations on a mutually beneficial basis. The joint venture "The Laboratory of cell transplantation surgery and human organ bio-engineering" will represent the interests of the head organisation abroad, provide information on the program and present this program abroad to attract additional investors on mutually beneficial parity basis.

    7. Control structure of the Program; supervision of its progress

    Structure of controlling the Program realisation consists of three levels:

    1. Controlling the Program realisation as a whole, co-ordination and scientific-methodical supervision.

    2. Co-ordination of projects included in the Program; making arrangements with the conjunct programs similar to it, such as "National base of technology", "High tech medicine", etc.

    3. Controlling the projects as a main program chains; making arrangements with the conjunct projects in the field of transplantation surgery and artificial organs.

    Controlling the Program realisation as a whole shall be carried out by the customers specifying the requirements to the Program's and projects' content in part of resource supply and progress control; and scientific leader who is responsible for the content and scientific-technical level of the Program and projects.

    The customers together with the Ministry of Health shall set up the joint venture "The Laboratory of cell transplantation surgery and human organ bio-engineering", which shall operate as a directorate to realise the international scientific research Program and shall be a co-ordinator and auditor of activity to implement the Program measures.

    The joint venture shall be headed directly by a person authorised by the Director of the Head organisation, the scientific research institute of transplantation surgery and artificial organs of the Ministry of Health of the Russian Federation. The Ministry of Health shall control over the Program realisation.

    Academician of the Russian Academy of Science and the Russian Academy of Medical Science Shumakov V.I. and professor Bryukhovetsky A.S. shall be scientific leaders of the Program; they shall develop the concept of the Program, its structures, methodical documents, co-ordinate the activities of scientific leaders of sub-programs, organise the preparation to scientific and practical conferences and scientific meetings on the subjects of the Program.

    The Program is controlled in accordance with the Order for development and realisation of federal purpose-oriented programs and inter-state purpose-oriented programs, in which the Russian Federation participates.

    8. Expected scientific-practical result of the Program and assessment of its efficiency

    1. On the theoretical basis of information approach in medicine, on the level of the world's best analogues, a smart, organ-saving, bioengineering, low-invasion, environmentally safe technology for the reconstruction of damaged organ tissue, the bio-engineering human organ plasty will be created and introduced in clinical practice, and scientific, methodical and experimental base to implement this new high medical technology will be formed.

    2. For the first time the scientifically substantiated engineering solutions will be worked out in form of specialist software allowing to provide guaranteed control of bio-technological measures, system designing and analysis of clinical-diagnostic criteria, dynamics of operative intervention and assessment of the efficiency of all the stages of healing process.

    3. For the first time in the world health practice the endovascular pharmacodynamic reconstruction of geometry and area of human brain vessels through program regional perfusion and high-push local pharmacological effect and restoration of vegetative innervation will be performed under the clinical conditions.

    4. For the first time to perform cell reconstruction and bio-assembly a versatile method of isolated cultivation of main cell elements of the brain tissue is developed within this technology and their program transplantation is made with strictly specified parameters of ratio of neurones and cells of neuro-glies.

    5. Bio-engineering restoration of damaged brain functions through the use of the developed technology with high economic effect of its use in central medical institutions of military and civil health is fixed as our country's priority.

    6. The first domestic culture bank of nerve cells is formed to perform cell transplantation and operations for bio-engineering organ plasty.

    7. For the first time in the world practice the transplantation of vascularized portion of chimer spinal marrow will be performed.

    8. Recommendations are worked out to introduce the obtained scientific and practical results into the routine of controlling bodies of highly specialised medical and prophylactic transplantation institutions of the Russian Federation.

    © 2000-2001

  4. #4

    Comments on the procedure

    Dimitriy (who frequently posted in the Cando forums) recently went to Moscow and received fetal transplants. From his description, I believe that the cells were transplanted via an intrathecal injection (i.e. the "Low invasive" approach). He described having had a headache after the procedure.

    Because the transplants are heterografts (i.e. from a genetically unrelated fetus), I was concerned about the possibility of immunological rejection of the cells. Apparently, the Russian groups have been testing patients before the procedure for the presence of antibodies against common central nervous system proteins (GFAP, neuron-specific enolase), to determine whether the patient is likely to have antibodies against transplanted neurons and glial cells. In some of the patients, they give a brief period of immunosuppression before the transplantation.

    The fact that they are using intrathecal injection of the cells is interesting because several studies have suggested that stem cells will migrate into the spinal cord when injected intrathecally into the cerebrospinal fluid or even intravascularly.


  5. #5
    See Posting "Dr.Bruhovetski's adress and phone/fax" in the Cure Forum for further information on the new clinic that Dr. Bruhovetski has established:

    [This message was edited by Wise Young on 07-28-03 at 07:59 PM.]

  6. #6
    Senior Member
    Join Date
    Mar 2004
    Warner Robins, GA, US
    I am interested in Dr. Bruhovetsky's low invasive procedure, but I would like to know more about it. Is there a way we can find out more about the results? What happened to Dimitri?

    gretchen 1

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