Stem Cell Rev Rep. 2019 Nov 23.

Intracerebroventricular Delivery of Human Umbilical Cord Mesenchymal Stem Cells as a Promising Therapy for Repairing the Spinal Cord Injury Induced by Kainic Acid.

Nishida F1,2, Zappa Villar MF2,3,4, Zanuzzi CN5,6,7, Sisti MS1,2, Cami?a AE1, Reggiani PC2,3,4, Portiansky EL1,2.

Author information
1
Image Analysis Laboratory, School of Veterinary Sciences, National University of La Plata (UNLP), Calles 60 y 118, 1900, La Plata, Buenos Aires, Argentina.
2
National Research Council of Science and Technology (CONICET), Buenos Aires, Argentina.
3
INIBIOLP, School of Medical Sciences, UNLP, La Plata, Buenos Aires, Argentina.
4
Department of Histology and of Embryology B, School of Medical Sciences, UNLP, La Plata, Buenos Aires, Argentina.
5
Image Analysis Laboratory, School of Veterinary Sciences, National University of La Plata (UNLP), Calles 60 y 118, 1900, La Plata, Buenos Aires, Argentina. carozanuzzi@fcv.unlp.edu.ar.
6
National Research Council of Science and Technology (CONICET), Buenos Aires, Argentina. carozanuzzi@fcv.unlp.edu.ar.
7
Department of Histology and Embryology, School of Veterinary Sciences, UNLP, La Plata, Buenos Aires, Argentina. carozanuzzi@fcv.unlp.edu.ar.
Abstract
Spinal cord injury (SCI) is a common pathological condition that leads to permanent or temporal loss of motor and autonomic functions. Kainic acid (KA), an agonist of kainate receptors, a type of ionotropic glutamate receptor, is widely used to induce experimental neurodegeneration models of CNS. Mesenchymal Stem Cells (MSC) therapy applied at the injured nervous tissue have emerged as a promising therapeutic treatment. Here we used a validated SCI experimental model in which an intraparenchymal injection of KA into the C5 segment of rat spinal cord induced an excitotoxic lesion. Three days later, experimental animals were treated with an intracerebroventricular injection of human umbilical cord (hUC) MSC whereas control group only received saline solution. Sensory and motor skills as well as neuronal and glial reaction of both groups were recorded. Differences in motor behavior, neuronal counting and glial responses were observed between hUC-MSC-treated and untreated rats. According to the obtained results, we suggest that hUC-MSC therapy delivered into the fourth ventricle using the intracerebroventricular via can exert a neuroprotective or neurorestorative effect on KA-injected animals.

KEYWORDS:
Astrocytes; Behavioral tests; Experimental SCI; Fourth ventricle; Microglia; Neurorestauration; Stem cell therapy

https://www.ncbi.nlm.nih.gov/pubmed/31760626