http://file.scirp.org/pdf/Health_2013081314523150.pdf
Vol.5, No.8A3, 9-15 (2013) Health http://dx.doi.org/10.4236/health.2013.58A3002
Copyright ? 2013 SciRes.


Effect of mango seed kernel extract on the adipogenesis in 3T3-L1 adipocytes and in rats fed a high fat diet
Mai Kobayashi1, Isao Matsui-Yuasa1, Maiko Fukuda-Shimizu2, Yoshinobu Mandai2, Masaki Tabuchi3, Hiroshi Munakata3, Akiko Kojima-Yuasa1*
1Department of Food and Human Health Sciences, Graduate School of Human Life Science, Osaka City University, Osaka, Japan *Corresponding Author: kojima@life.osaka-cu.ac.jp
2Nitta Gelatin Inc., Yao, Japan
3Department of Biochemistry, Kinki University Medical School, Osaka-Sayama, Japan
Received 22 June 2013; revised 15 July 2013; accepted 26 July 2013
Copyright ? 2013 Mai Kobayashi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


ABSTRACT
Mangoes (Mangifera indica L.) are one of the most important tropical foods. The seed is one of the main by-products of mango processing. Therefore, it is important to find an economically viable use for this waste (e.g., as a food additive or supplement with high nutraceutical value). We investigated the anti-obesity effects of man- go seed kernel extract with hot water (MSKE-W) in 3T3-L1 adipocytes and in a high fat diet (HFD)-induced obesity rat model. MSKE-W cau- sed a significant decrease in the activity of gly- cerol 2-phosphate dehydrogenase in 3T3-L1 adi- pocytes without eliciting cell cytotoxicity and inhibited cellular lipid accumulation through down-regulation of transcription factors such as PPARγ and C/EBPα. In the animal model, rats fed an HFD containing 1% MSKE-W gained less weight than rats fed an HFD alone. The visceral fat mass in rats fed an HFD containing 1% MSKE-W tended to be lower than that in rats fed an HFD alone. Furthermore, histological exami- nation of rat livers from an HFD showed steato- hepatitis. However, rats on an HFD containning 1% MSKE-W showed no histopathological chan- ges in liver tissue. Our results indicate that MSKE-W influences anti-obesity effects, both in vitro and in vivo, and suggest that MSKE-W provides a novel preventive potential against obesity.
Proliferator-Activated Receptor γ (PPARγ); High Fat Diet-Induced Obesity Rat Model; 3T3-L1 Adipocytes