J Biol Chem. 2010 Feb 5.

Generation of human induced pluripotent stem cells from umbilical cord matrix and amniotic membrane mesenchymal cells.

Cai J, Li W, Su H, Qin D, Yang J, Zhu F, Xu J, He W, Guo H, Labuda K, Peterbauer A, Wolbank S, Zhong M, Li Z, Wu W, So KF, Redl H, Zeng L, Esteban MA, Pei D.

GIBH, China;

The umbilical cord and placenta are extraembryonic tissues of particular interest for regenerative medicine. They share an early developmental origin and are source of vast amounts of cells with multi-lineage differentiation potential that are poorly immunogenic and exempt of controversy. Here we report the efficient nuclear reprogramming using exogenous factors of mesenchymal cells from placental chorionic and amniotic membranes and the umbilical cord. Induced pluripotent stem cells (iPSC) from all 3 sources homogeneously showed human embryonic stem cell (ESC)-like characteristics including morphology, normal karyotype, positive staining for alkaline phosphatase, and high expression levels of ESC-like markers (including Nanog, Rex1, Oct4, TRA-1-60, TRA-1-80, SSEA-3, and SSEA-4). Selected iPSC also formed teratomas composed of the 3 germ layers and readily produced functional motorneurons upon induced differentiation. We conclude that the umbilical cord and placenta can be used to produce high quality iPSC. These cell lines may prove useful to compare the epigenetic modifications, differentiation ability, and stability of the resulting lineages, between iPSC derived from multiple tissues.

http://www.ncbi.nlm.nih.gov/pubmed/2...&ordinalpos=13