Tissue Eng Part A. 2010 Feb 10.

Generation of induced pluripotent stem cells from human adipose-derived stem cells without c-MYC.

Aoki T, Ohnishi H, Oda Y, Tadokoro M, Sasao M, Kato H, Hattori K, Ohgushi H.

Shinshu University School of Medicine, Department of Orthopedic Surgery, Matsumoto, Japan; tetsuhiro-aoki@aist.go.jp.

Human adipose-derived stem cells (hASCs) are ubiquitous, plentiful, and easily/safely obtainable cells derived from adipose tissue, regardless of the age and sex of the donor. However, the hASCs have limited proliferative and differentiation capabilities. In this study, we examined whether induced pluripotent stem cells (iPSCs) could be generated from hASCs. We transduced hASCs with three human transcription factors (OCT3/4, SOX2, and KLF4), and found that they formed human embryonic stem cell (ESC)-like colonies. Importantly we did not transduce c-MYC, which is usually utilized to generate iPSCs but is considered an oncogene. These colonies expressed human ES cell-specific surface antigens (stage-specific embryonic antigens SSEA-3, SSEA-4, tumor-related antigens TRA-1-60 and TRA-1-81), endogenous transcription factors (OCT3/4, NANOG, SOX2), and undifferentiated human ES cell maker genes (REX1, UTF1, GDF3, DPPA2, DPPA4, and DPPA5). Furthermore, the colonies were able to differentiate into the three germ layers both in vitro and in vivo. These results show that human iPSCs can be generated by the transduction of three factors (OCT3/4, SOX2, and KLF4) into hASCs.