[Treatment of spinal cord injury by transplanting neural stem cells with NgR gene silencing]

Zhongguo Wei Zhong Bing Ji Jiu Yi Xue. 2010 Jan;22(1):28-31.

[Article in Chinese]
Wang D, Zhang JJ, Yang ZX.

Department of Neurosurgery, Tianjin Fourth Central Hospital, Tianjin 300140, China.

OBJECTIVE: To determine whether Nogo-66 receptor (NgR) gene silencing in neural stem cells (NSCs) can ameliorate spinal cord injury (SCI) in rats. METHODS: The brain tissue of Wistar rats of embryo (age 14-16 days) was obtained, and NSCs were cultured in suspension culture, and they were transfected by siRNA to silence the expression of NgR. Western blotting was used to assess the silencing efficiency. Thirty-six Wistar rats were divided randomly into three groups. Hemi-truncation of right-half side of 8, 9 thoracic spinal cord was performed. Group A was injected same amount of culture medium without NSCs, B group was given naive NSCs suspension, and C group same amount of NgR gene silenced NSCs. At 1, 2, 4, 6, 8 weeks post-injury, the motor power of the hind limbs of all animals were evaluated with inclined plane test. continue...