Results 1 to 6 of 6

Thread: Publication about Dr.Huang's OECs transplantation

  1. #1

    Publication about Dr.Huang's OECs transplantation

    A search from PubMed about Dr.Huang’s publication



    1, Chin Med J (Engl). 2003 Oct;116(10):1488-91
    Influence of patients' age on functional recovery after transplantation of olfactory ensheathing cells into injured spinal cord injury.
    Huang H, Chen L, Wang H, Xiu B, Li B, Wang R, Zhang J, Zhang F, Gu Z, Li Y, Song Y, Hao W, Pang S, Sun J.
    Department of Neurosurgery, Chaoyang Hospital, Capital University of Medical Sciences, Beijing 100020, China. hongyun@mailcity.com
    OBJECTIVE: To evaluate the restoration of function after spinal cord injury (SCI) in patients of different ages who have underwent intraspinal transplantation of olfactory ensheathing cells (OECs). METHODS: One hundred and seventy-one SCI patients were included in this study. Of them, 139 were male and 32 were female, with age ranging from 2 to 64 years (mean, 34.9 years). In all SCI patients the lesions were injected at the time of operation with OECs. According to their ages, the patients were divided into 5 groups: </= 20 years group (n = 9), 21 - 30 years group (n = 54), 31 - 40 years group (n = 60), 41 - 50 years group (n = 34) and > 51 years group (n = 14). The spinal cord function was assessed based on the American Spinal Injury Association (ASIA) Classification System before and 2 - 8 weeks after OECs transplantation. One-way ANOVA and q test were used for statistical analysis, and the data were expressed as mean +/- SD. RESULTS: After surgery, the motor scores increased by 5.2 +/- 4.8, 8.6 +/- 8.0, 8.3 +/- 8.8, 5.7 +/- 7.3 and 8.2 +/- 7.6 in 5 age groups respectively (F = 1.009, P = 0.404); light touch scores increased by 13.9 +/- 8.1, 15.5 +/- 14.3, 12.0 +/- 14.4, 14.1 +/- 18.5 and 24.8 +/- 25.3 respectively (F = 1.837, P = 0.124); and pin prick scores increased by 11.1 +/- 7.9, 17.2 +/- 14.3, 13.2 +/- 11.8, 13.6 +/- 13.9 and 25.4 +/- 24.3 respectively (F = 2.651, P = 0.035). Restoration of pin prick in > 51 years group was better than other age groups except 21 - 30 years group. CONCLUSION: OECs transplantation can improve the neurological function of spinal cord of SCI patients regardless of their ages. Further research into the long-term outcomes of the treatment will be required.
    PMID: 14570607 [PubMed - indexed for MEDLINE]
    http://www.ncbi.nlm.nih.gov/pubmed/14570607?ordinalpos=37&itool=EntrezSystem2.PEntrez .Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum

  2. #2

    Influence factors for functional improvement after olfactory ensheathing cell transplantation for chronic spinal cord injury.

    Neurological Research and Treatment Center, Beijing Xishan Hospital, PR China. hongyun@mailcity.com
    OBJECTIVE: To explore the influence factors for the functional improvement after the fetal olfactory ensheathing cell (OEC) transplantation for chronic spinal cord injury(SCI). METHODS: The olfactory bulbs were harvested and trypsinized down to single fetal OEC. They were cultured for 12-17 days prepared for use. From November 2001 to December 2003, a total of 300 patients volunteered for the fetal OEC transplantation, among whom 222 suffered from complete chronic SCI and 78 suffered from incomplete chronic SCI. The procedures were performed on the patients with a disease course ranging from 6 months to 31 years (average 3.1 years) after their injuries. The fetal OEC was transplanted by the form of injections into the spinal cord at the upper and lower ends of the injury site. All the patients were assessed by the ASIA standard before the transplantation and 2-8 weeks after the transplantation. The influence factors including age, sex, duration after the injury, and injury degrees and levels were compared with those in the functional improvement after fetal OEC transplantation. RESULTS: The partially-improved neurological functions assessed by the ASIA standard were indicated by the motor scores increasing from 39.1 +/- 20.6 to 45.9 +/- 20.3 (P < 0.001), the light touch scores from 51.7 +/- 24.9 to 63.4 +/- 23.0 (P < 0.001), and the pin prick scores from 53.0 +/- 24.2 to 65.3 +/- 22.7 (P < 0.001). There was no significant difference in the functional improvement of the motor, light touch, and pin brick when compared with the age, sex, duration after the injury, and the injury degrees and levels. The motor scores and light touch scores at the cervical level were higher than the scores at the thoracic level. CONCLUSION: The fetal OEC transplantation can partially improve the neurological functions quickly in treatment of the chronic spinal cord injury. All the influence factors except the motor scores and light touch scores, which were higher at the cervical level than at thoracic level, have no impact on the functional improvement after the fetal OEC transplantation.
    PMID: 16683451 [PubMed - in process]






    http://www.ncbi.nlm.nih.gov/pubmed/16683451?ordinalpos=25&itool=EntrezSystem2.PEntrez.Pubmed.P ubmed_ResultsPanel.Pubmed_RVDocSum
    Last edited by ndassoc; 08-07-2008 at 05:21 AM.

  3. #3
    3, Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2006 Apr;20(4):439-43
    Safety of fetal olfactory ensheathing cell transplantation in patients with chronic spinal cord injury. A 38-month follow-up with MRI.
    Huang H, Chen L, Wang H, Xi H, Gou C, Zhang J, Zhang F, Liu Y.
    Neurological Research and Treatment Center, Beijing Xishan Hospital, Beijing, 100041, PR China. hongyun@mailcity.com
    OBJECTIVE: To determine the safety of the fetal olfactory ensheathing cell (OEC) transplantation in patients with chronic spinal cord injury (SCI) by examination of the magnetic resonance imaging (MRI). METHODS: A prospective clinical study involving 16 patients with chronic SCI was designed to investigate the feasibility and biological safety of the fetal OEC transplantation in treatment of SCI. The olfactory bulbs from the 3-4-month-old aborted human fetuses following the strict ethical guidelines were harvested and trypsinized down to single fetal OEC. These cells were then cultured for 12-17 days and were prepared for a clinical use. From November 2001 to December 2002, 16 patients with chronic SCI were randomly enrolled. The patients suffered from SCI for 1.5-8 years (average 4.3 years) after the injury. The suspension (50 microl) containing about 1 x 10(6) fetal OECs was transplanted by an injection into the patients' spinal cords above and below the injury site. All the patients were assessed before the transplantation and were followed up with MRI for 29-42 months (average 38 mon) after the transplantation. RESULTS: No cell-related adverse effects were observed in any patient during the follow-up period. The follow-up with MRI did not reveal any development of optic glial tumor, tumor-like mass, new hemorrhage, edema, expanding cyst, new cyst formation, infection or disruption of the neural structure in the transplant site of all the patients. CONCLUSION: This is the first clinical study demonstrating the long-term safety of the OEC therapy for SCI. The results indicate that our protocol is feasible and safe in treatment of patients with chronic SCI within 38 months after the injury. Although the size of the samples for our study was not big enough, the positive results of the study have encouraged us to make a further research in this field.
    PMID: 16683452 [PubMed - in process http://www.ncbi.nlm.nih.gov/pubmed/16683452?ordinalpos=24&itool=EntrezSystem2.PEntrez .Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum

  4. #4
    4, Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2007 Jan;21(1):52-7
    MR spectroscopy evaluation and short-term outcome of olfactory ensheathing cells transplantation in amyotrophic lateral sclerosis patients.
    Huang H, Tan K, Chen L, Xue Y, Wang H, Xi H, Liu Y, Zhang F, Zhang J.
    Beijing Xishan Institute for Neuroregeneration and Functional Recovery in Shijingshan District, Beijing, P. R. China. hongyun@mailcity.com
    OBJECTIVE: To evaluate proton MR spectroscopy (1H-MRS) for detection of the motor cortex and adjacent brain in amyotrophic lateral sclerosis (ALS) patients with apparent upper motor neuron involvement after olfactory ensheathing cells (OECs) transplantation. METHODS: From December 2004 to February 2005, 7 patients with clinically definite ALS who could safely undergo MRS were admitted into the perspective study. The neurological status, ALS functional rating scale (ALSFRS), EMG, and 1H-MRS taken before and 2 weeks after operations were carefully analyzed. The NAA/Cr and Cho/Cr ratios were measured in the cerebral peduncle, genu and posterior limb of the internal capsule, corona radiata and precentral gyrus. RESULTS: The ALSFRS in 2 cases improved obviously whose ALSFRS increased from 30 to 33 and from 29 to 34 respectively. And 5 cases remained stable 2 weeks after OECs transplantation. Statistical analyses for all seven cases showed both the NAA/Cr and Cho/Cr ratios decreased, but in the two cases with ALSFRS improvement the NAA/Cr increased in the certain anatomic position which confirmed the neurological and EMG findings. CONCLUSION: The proton MR spectroscopy is a suitable noninvasive measure for ALS evaluation. The preliminary study suggests that two of the seven ALS cases improved apparently short-term after OECs transplantation. More patients are required for the clinical study and longer follow-up duration is needed for future research.
    PMID: 17305006 [PubMed - in process]
    http://www.ncbi.nlm.nih.gov/pubmed/17305006?ordinalpos=16&itool=EntrezSystem2.PEntrez .Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum

  5. #5
    5, Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2007 Sep;21(9):961-6.
    Short-term outcome of olfactory ensheathing cells transplantation for treatment of amyotrophic lateral sclerosis.
    Chen L, Huang H, Zhang J, Zhang F, Liu Y, Xi H, Wang H, Gu Z, Song Y, Li Y, Tan K.
    Beijing Xishan Institute for Neuroregeneration and Functional Recovery in Shijingshan District, Beijing 100041, PR China. chenlin_china@hotmail.com
    OBJECTIVE: To determine whether transplanting olfactory ensheathing cells (OECs) is effective in controlling or reversing the deterioration caused by amyotrophic lateral sclerosis (ALS). METHODS: Between February 2003 and April 2006, 327 patients (241 males and 86 females) with probable or definite ALS (diagnosed according to the El Escorial criteria) received the OECs transplantation. Their ages ranged from 20 to 84 years (51.6 +/- 11.1 years). The duration of symptoms before surgical treatment was 4.8 months to 13 years (2.9 +/- 2.0 years). OECs were cultured and injected into pathological regions of the spinal cord and/or bilateral corona radiata of the brain; the patients were divided into three groups, group A (cord only, n = 29), group B (cord and brain, n = 6), and group C (brain only, n = 292) based on the transplant sites. RESULTS: The patient's neurological function was assessed both before and at 4 weeks after transplantation by using the Amyotrophic Lateral Sclerosis Functional Rating Scale (ALSFRS) of the ALS CNTF Treatment Study (ACLS). The scores were increased from 17.2 +/- 8.6 pre-operation to 20.1 +/- 9.7 post-operation in group A (P < 0.05), from 24.2 +/- 6.8 to 25.7 +/- 6.6 (P > 0.05) in group B, and from 20.3 +/- 8.6 to 22.0 +/- 9.4 (P < 0.001) in group C. There were no significant difference in increased ALSFRS scores among the three groups (P > 0.05). The total improvement rate of neurological function was 77.1% (252/327). The result of electromyographic examination showed that spontaneous potential diminished and/or disappeared, the amplitude of the motor unit action potential decreased remarkably and the numbers of motor unit action potential greatly increased in 261 cases (79.8%). Sixteen patients (4.9%) experienced the various complications including headache, short-term fever, seizure attack, central nerve system infection, pneumonia, respiratory failure, urinary tract infection, heart failure, and possible pulmonary embolism; of them, there were 4 deaths (1.2%). CONCLUSION: These preliminary results suggest that the OECs transplantation is effective in controlling or reversing the physiological deterioration caused by ALS.
    PMID: 17933231 [PubMed - in process]
    http://www.ncbi.nlm.nih.gov/pubmed/17933231?ordinalpos=12&itool=EntrezSystem2.PEntrez .Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum

  6. #6
    6, Clin Transplant. 2008 Jul 31. [Epub ahead of print]
    Fetal olfactory ensheathing cells transplantation in amyotrophic lateral sclerosis patients: a controlled pilot study.
    Huang H, Chen L, Xi H, Wang H, Zhang J, Zhang F, Liu Y.
    Beijing Hongtianji Neuroscience Academy, and Department of Neurosurgery, Beijing Xishan Hospital, Beijing, China.
    This study was designed to clarify whether transplantation of fetal olfactory ensheathing cells (OECs) would affect the clinical course of patients with amyotrophic lateral sclerosis (ALS). Thirty-five patients with probable or definite ALS were enrolled from December 2004 to September 2006; 15 patients received OECs transplantation and 20 patients did not receive OECs transplantation. OECs were cultured and injected into the bilateral corona radiata involving the pyramidal tracts of the frontal lobes. The primary end point used to indicate effectiveness was the rate of change according to the ALS Functional Rating Scale (ALS-FRS) total score. All patients were tested five times at baseline and monthly intervals during a four-month follow-up period using assessment of ALS-FRS. Thirty-one patients (14 in the OECs treated group and 17 in the controls) completed the four-month study; the remaining four patients were lost to follow-up. Patients' data were analyzed four months after OECs transplantation and at the end of the controlled period. There was no significant difference in the rate of progression as measured by the ALS-FRS total score during the first two months (p > 0.05). The functional deterioration, however, was significantly slower in the treated group than in the control group during the last two months (p < 0.05). The mean (+/-SD) change for the ALS-FRS total score was 0.07 +/- 4.18 for the treated group and 6.12 +/- 5.49 for the control group (p = 0.002) during the four months. Of the 14 patients in the treatment group, seven experienced neurological functional improvements, two were stable compared with their clinical status at entry, and the ALS-FRS scores in the other five decreased by a mean of 4.4. Of the 17 patients in the control group, only one patient's condition remained stable while the ALS-FRS scores in the other 16 decreased by a mean of 6.5. The result indicates OECs transplantation appears to be able to slow the rate of clinical progression of ALS in the first four months posttransplantation.
    PMID: 18673377 [PubMed - as supplied by publisher]
    http://www.ncbi.nlm.nih.gov/pubmed/18673377?ordinalpos=1&itool=EntrezSystem2.PEntrez. Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum

Similar Threads

  1. Replies: 21
    Last Post: 12-02-2004, 04:01 PM
  2. Replies: 2
    Last Post: 08-06-2003, 06:11 PM
  3. OEG question
    By mk99 in forum Cure
    Replies: 7
    Last Post: 05-26-2003, 02:52 PM
  4. Replies: 17
    Last Post: 04-13-2003, 03:26 PM
  5. LANCET´S ARTICLE
    By KIM in forum Cure
    Replies: 2
    Last Post: 10-04-2002, 09:52 AM

Posting Permissions

  • You may not post new threads
  • You may not post replies
  • You may not post attachments
  • You may not edit your posts
  •