Wise Young
01-07-2002, 07:26 AM
Reference Type: Journal Article
Record Number: 875
Author: Ming, G.; Henley, J.; Tessier-Lavigne, M.; Song, H.; Poo, M.
Year: 2001
Title: Electrical activity modulates growth cone guidance by diffusible factors
Journal: Neuron
Volume: 29
Issue: 2
Pages: 441-52
Date: Feb
Accession Number: 11239434
Keywords: Animal
Calcium/metabolism/*physiology
Cells, Cultured
Chemotaxis
Cyclic AMP/metabolism/physiology
Electric Stimulation
Glycoproteins/*pharmacology
Growth Cones/*drug effects/physiology
Myelin Sheath/metabolism/*physiology
Myelin-Associated Glycoprotein/*pharmacology
Nerve Growth Factors/*pharmacology
Nerve Regeneration/drug effects/physiology
Neurons/drug effects/physiology
Support, Non-U.S. Gov't
Support, U.S. Gov't, P.H.S.
Xenopus
Abstract: Brief periods of electrical stimulation of cultured Xenopus spinal neurons resulted in a marked alteration in the turning responses of the growth cone induced by gradients of attractive or repulsive guidance cues. Netrin-1-induced attraction was enhanced, and the repulsion induced by myelin-associated glycoprotein (MAG) or myelin membrane fragments was converted to attraction. The effect required the presence of extracellular Ca(2+) during electrical stimulation and appeared to be mediated by an elevation of both cytoplasmic Ca(2+) and cAMP. Thus, electrical activity may influence the axonal path finding of developing neurons, and intermittent electrical stimulation may be effective in promoting nerve regeneration after injury.
Notes: 0896-6273
Journal Article
URL: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=11239434
Author Address: Department of Biology, University of California, San Diego, La Jolla, CA 92093, USA.
Record Number: 875
Author: Ming, G.; Henley, J.; Tessier-Lavigne, M.; Song, H.; Poo, M.
Year: 2001
Title: Electrical activity modulates growth cone guidance by diffusible factors
Journal: Neuron
Volume: 29
Issue: 2
Pages: 441-52
Date: Feb
Accession Number: 11239434
Keywords: Animal
Calcium/metabolism/*physiology
Cells, Cultured
Chemotaxis
Cyclic AMP/metabolism/physiology
Electric Stimulation
Glycoproteins/*pharmacology
Growth Cones/*drug effects/physiology
Myelin Sheath/metabolism/*physiology
Myelin-Associated Glycoprotein/*pharmacology
Nerve Growth Factors/*pharmacology
Nerve Regeneration/drug effects/physiology
Neurons/drug effects/physiology
Support, Non-U.S. Gov't
Support, U.S. Gov't, P.H.S.
Xenopus
Abstract: Brief periods of electrical stimulation of cultured Xenopus spinal neurons resulted in a marked alteration in the turning responses of the growth cone induced by gradients of attractive or repulsive guidance cues. Netrin-1-induced attraction was enhanced, and the repulsion induced by myelin-associated glycoprotein (MAG) or myelin membrane fragments was converted to attraction. The effect required the presence of extracellular Ca(2+) during electrical stimulation and appeared to be mediated by an elevation of both cytoplasmic Ca(2+) and cAMP. Thus, electrical activity may influence the axonal path finding of developing neurons, and intermittent electrical stimulation may be effective in promoting nerve regeneration after injury.
Notes: 0896-6273
Journal Article
URL: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=11239434
Author Address: Department of Biology, University of California, San Diego, La Jolla, CA 92093, USA.