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View Full Version : Safety, Tolerability & Effects of Rolipram on Inflammatory Activity in the Central Nervous System in Multiple Sclerosis. A Phase II, Open Label Crossover Trial Using MRI as an Outcome Measure


Wise Young
08-06-2001, 01:38 AM
http://clinicaltrials.gov/ct/gui/c/a1b/show/NCT00011375?order=38&JServSession Idzone_ct=zhn5b9gan1 (http://clinicaltrials.gov/ct/gui/c/a1b/show/NCT00011375?order=38&JServSessionIdzone_ct=zhn5b9gan1)

Rolipram to Treat Multiple Sclerosis

This study is currently recruiting patients.

Sponsored by

National Institute of Neurological Disorders and Stroke (NINDS)

Purpose

This study will evaluate the safety, tolerability, and effect of the drug Rolipram on multiple sclerosis (MS). It will examine whether Rolipram can dampen the part of the immune response believed to lead to MS and reduce disease activity.

Patient with multiple sclerosis who are between the ages of 18 and 65 may be eligible for this study. Candidates will be screened with a complete neurological and medical evaluation. Participants will complete three study phases-baseline, treatment and follow-up, as follows:

Baseline (3 months) < Approximately four magnetic resonance imaging (MRI) scans will be obtained to assess MS activity. Participants with MS activity above a certain level will continue with the treatment phase.

Treatment (8 months) < Patients will take Rolipram tablets in increasing doses every 2 to 3 days for the first month of this phase until their individual maximum tolerated dose is established. Dosing will continue at that level for the rest of the treatment phase. Dosing is in the morning, midday and evening. Patients will be seen monthly in the clinic for examination and MRI scans.

Follow-up < Participants will have monthly exams and MRIs for 3 months following the treatment phase, after which their participation in the study ends.

Patients' monthly visits during treatment and follow-up include a neurological examination to assess disease status; MRI to assess brain changes; and blood and urine collection to monitor liver, kidney and other functions. In addition, a lumbar puncture (spinal tap) is done during the last month of the baseline phase and one month after treatment ends to study changes in the spinal fluid surrounding the brain and spinal cord, and leukapheresis is done once during the last month of the baseline phase and once during the last month of treatment to collect white blood cells for study. These procedures involve the following:

MRI uses a strong magnetic field and radio waves instead of X-rays to produce images showing structural and chemical changes in tissues. The patient lies on a table in a narrow cylinder (the scanner) containing a magnetic field and images are taken. A contrast agent called gadolinium is injected into a vein during the last set of images to help identify new lesions. Magnetic resonance spectroscopy, which is similar to MRI, is also done once during the baseline phase, at 4 months and at 8 months to measure brain chemicals. For the spinal tap, a local anesthetic is given and a needle is inserted in the space between the bones (vertebrae) in the lower back. About 2 tablespoons of fluid is collected through the needle. For leukapheresis, whole blood is collected through a needle placed in an arm vein. The blood circulates through a machine that separates it into its components. The white cells are removed and the red cells, platelets and plasma are returned to the body through a second needle placed in the other arm.

Patients may also have studies to measure levels of Rolipram in the blood. These are done on study days 1 and 29 and at months 2, 4, and 6. For days 1 and 29, a catheter is placed in an arm vein and 4 ml. of blood is drawn immediately before the morning dose and at several intervals from 20 minutes to 6 hours after the dose. For the other tests, a single 4-ml sample is collected before the noon dose.

Condition Treatment or Intervention Phase Multiple Sclerosis

Drug:Rolipram

Phase II

MEDLINEplusrelated topics:MultipleSclerosis

Study Type:Clinical Trial


Official Title:Safety, Tolerability & Effects of Rolipram on Inflammatory Activity in the Central Nervous System in Multiple Sclerosis. A Phase II, Open Label Crossover Trial Using MRI as an Outcome Measure

Further Study Details:

Rolipram is a phosphodiesterase (PDE) type 4 inhibitor that has originally been developed by Schering AG, Berlin, Germany, as an antidepressant, before others and our laboratory documented the immunomodulatory properties of the drug. In the current trial, Rolipram will for the first time be tested as a novel immunomodulatory therapy in multiple sclerosis patients. The protocol involves a stage I for finding the highest individually well-tolerated drug dose, before stage II, and 8 months treatment period with this individually well-tolerated dose, will be conducted. The trial shall document the safety, tolerability an efficacy with respect to inhibition of central nervous inflammation in multiple sclerosis patients. Magnetic resonance imaging and clinical examinations will be used to study the above parameters, and immunological studies that will be conducted in parallel to the trial, will address the mechanism of action of Rolipram in MS.

Eligibility

Genders Eligible for Study: Both

Criteria

Must be between the ages of 18 and 65 years. Must have clinically definite relapsing-remitting or secondary progresssive MS according to published criteria. Must have EDSS score between 4.0 and 6.5 for stage I and be between 1.5 and 6.5 for stage II. Patients must have either failed standard treatment (interferon beta, glatiramer acetate) by clinical measures and/or were not eligible for ay of the standard treatments available or opted not to start or to continue with any of these treatments. Must have an average of up to 2 Gd-enhancing lesions per month over the 3 month pre-treatment baseline period for stage I and an average of at least 0.5 Gd-enhancing lesions per month over the 4 month pre-treatment period for stage II. Subjects must not have a relapse during 30 days before initiation of treatment. If a relapse occurs during the last 30 days of the pre-treatment baseline period and eligibility MRI criteria are fulfilled, beginning of the treatment (day 1) is delayed for at least so many days that treatment starts not earlier than 60 days in case I.V. corticosteroids had been given. Similarly, the baseline needs to be prolonged if corticosterioid treatments become necessary dring these three months. Patients will be excluded from study entry if any of the following exclusion criteria exist at the time of enrollment. If a washout period of previous treatments becomes necessary, thse criteria have to be assessed again at the beginning of the MRI baseline period. An additional assessment of the exclusion criteria follows on day 0 of the 6 months treatmnet phase.


Location and Contact Information

Maryland
National Institute of Neurological Disorders and Stroke (NINDS),9000 Rockville Pike Bethesda, Maryland, 20892, United States;Recruiting

PRPL 1-800-411-1222 prpl@mail.cc.nih.gov

More Information

Detailed Web Page

Publications

Sommer. 1995. The antidepressant rolipram suppresses cytokine production and prevents autoimmune encephalomyelitis, Nature Med, Vol. 1995, p. 244

Hasko. 1998. Supression of IL-12 production by phosphodiesterase inhibition in murine endotoxemia is IL-10 independent, Eur J Immunol, Vol. 28, p. 468

Pette. 1999. The immunomodulatory profile of phosphodiesterase type 4-specific inhibition of human autoreactive T cell clones: Basis for the treatment of T helper 1 lymphocytes-mediated autoimmune diseases, Neuroimmunol, Vol. 98, p. 147

Study ID Numbers 01-N-0089
NLM Identifier NCT00011375

Date study startedFebruary 12, 2001
Record last reviewed January 25, 2001
Last Updated January 25, 2001

[This message was edited by Wise Young on August 06, 2001 at 12:32 PM.]

Wise Young
04-06-2002, 12:46 PM
updated links:
clinicaltrials.gov link (http://clinicaltrials.gov/ct/gui/c/a1r/show/NCT00011375?order=30&JServSessionIdzone_ct=nh9kvtwwa1)

Details:
http://clinicalstudies.info.nih.gov/detail/A_2001-N-0089.html

Protocol Number: 01-N-0089

Title:
Safety, Tolerability & Effects of Rolipram on Inflammatory Activity in the Central Nervous System in Multiple Sclerosis. A Phase II, Open Label Crossover Trial Using MRI as an Outcome Measure

Number:
01-N-0089

Summary:
This study will evaluate the safety, tolerability, and effect of the drug Rolipram on multiple sclerosis (MS). It will examine whether Rolipram can dampen the part of the immune response believed to lead to MS and reduce disease activity.

Patient with multiple sclerosis who are between the ages of 18 and 65 may be eligible for this study. Candidates will be screened with a complete neurological and medical evaluation. Participants will complete three study phases-baseline, treatment and follow-up, as follows:

Baseline (3 months) - Approximately four magnetic resonance imaging (MRI) scans will be obtained to assess MS activity. Participants with MS activity above a certain level will continue with the treatment phase.

Treatment (8 months) - Patients will take Rolipram tablets in increasing doses every 2 to 3 days for the first month of this phase until their individual maximum tolerated dose is established. Dosing will continue at that level for the rest of the treatment phase. Dosing is in the morning, midday and evening. Patients will be seen monthly in the clinic for examination and MRI scans.

Follow-up - Participants will have monthly exams and MRIs for 3 months following the treatment phase, after which their participation in the study ends.

Patients' monthly visits during treatment and follow-up include a neurological examination to assess disease status; MRI to assess brain changes; and blood and urine collection to monitor liver, kidney and other functions. In addition, a lumbar puncture (spinal tap) is done during the last month of the baseline phase and one month after treatment ends to study changes in the spinal fluid surrounding the brain and spinal cord, and leukapheresis is done once during the last month of the baseline phase and once during the last month of treatment to collect white blood cells for study. These procedures involve the following:

MRI uses a strong magnetic field and radio waves instead of X-rays to produce images showing structural and chemical changes in tissues. The patient lies on a table in a narrow cylinder (the scanner) containing a magnetic field and images are taken. A contrast agent called gadolinium is injected into a vein during the last set of images to help identify new lesions. Magnetic resonance spectroscopy, which is similar to MRI, is also done once during the baseline phase, at 4 months and at 8 months to measure brain chemicals. For the spinal tap, a local anesthetic is given and a needle is inserted in the space between the bones (vertebrae) in the lower back. About 2 tablespoons of fluid is collected through the needle. For leukapheresis, whole blood is collected through a needle placed in an arm vein. The blood circulates through a machine that separates it into its components. The white cells are removed and the red cells, platelets and plasma are returned to the body through a second needle placed in the other arm.

Patients may also have studies to measure levels of Rolipram in the blood. These are done on study days 1 and 29 and at months 2, 4, and 6. For days 1 and 29, a catheter is placed in an arm vein and 4 ml. of blood is drawn immediately before the morning dose and at several intervals from 20 minutes to 6 hours after the dose. For the other tests, a single 4-ml sample is collected before the noon dose.

Sponsoring Institute:
National Institute of Neurological Disorders and Stroke (NINDS)


Recruitment Detail
Type: Active Accrual Of New Subjects
Gender: Male & Female


Referral Letter Required: No

Population Exclusion(s): Children

Eligibility Criteria:

Inclusion Criteria for Pre-Treatment Screening:

Patients must be between the ages of 18 and 65 years, inclusive.


Subjects must have clinically definite relapsing-remitting or secondary progresssive MS according to published criteria.


Patients must have EDSS score between 4.0 and 6.5 for stage I and be between 1.5 and 6.5 for stage II.


Patients must have either failed standard treatment (interferon beta, glatiramer acetate) by clinical measures and/or were not eligible for ay of the standard treatments available or opted not to start or to continue with any of these treatments.


Eligiblity Criteria For Initiating Therapy:


Stage I:


Subjects must have an average of up to 2 Gd-enhancing lesions per month over the 3 month pre-treatment baseline period (i.e. over 4 MRI scans).


Stage II:


Subjects must have an average of at least 0.5 Gd-enhancing lesions per month over the 4 month pre-treatment baseline period.


Stage I & II:


Subjects must not have a relapse during 30 days before initiation of treatment. If a relapse occurs during the last 30 days of the pre-treatment baseline period and eligibility MRI criteria are fulfilled, beginning of the treatment (day 1) is delayed for at least so many days that treatment starts not earlier than 30 days after the relapse and not earlier than 60 days in case i.v. corticosteroids had been given. Similarly, the baseline needs to be prolonged if corticosterioid treatments become necessary dring these three months.


Patients will be excluded from study entry if any of the following exclusion criteria exist at the time of enrollment. If a washout period of previous treatments becomes necessary, these criteria have to be assessed again at the beginning of the MRI baseline period. An additional assessment of the exclusion criteria follows on day 0 of the 6 months treatmnet phase.


Blood tests exceeding any of the limits defined below will lead to exclusion from the study: ALT (SGPT) or AST (SGOT) is greater than three times the upper limit of normal; total white blood cell count is less than 3,000/mm(3), neutrophils less than 2,000; platelet count of less than 85, 000/mm(3); creatinine greater than 1.5 mg/dl; serology indicating HIV infection or active hepatitis A, B, or C infection; postive pregnancy test or breast-feeding female; nausea/vomiting as a frequent complaint; fasting prolactin levels of greater than 20 micrograms/L; history or signs of immunodeficiency; history of galactorrhea and/or history of prolactin-secreting tumors, history of pituitary tumors and adenomas, history of mammary tumors; clinically relevant arrhythmia or other ECG abnormalities.


Clinically significant (as determined by the investigator) cardiac, immunological, pulmonary, neurological, renal, and/or other major disease including ECG, echocardiogram, and chest x-ray examinations will be excluded.


Patients with mental impairment that would compromise understanding and judging the consent will be excluded from the study.


Special Instructions: Currently Not Provided

Keywords:
Phosphodiesterase Inhibitor
Immunology
Immunomodulation
Antidepressant
Therapy


Recruitment Keywords:
Multiple Sclerosis
Immunotherapy


Conditions:
Multiple Sclerosis


Investigational Drug(s):
Rolipram


Investigational Device(s):
None

Contacts:
Patient Recruitment and Public Liaison Office
Building 61
10 Cloister Court
Bethesda, Maryland 20892-4754
Toll Free: 1-800-411-1222
TTY: 301-594-9774 (local),1-866-411-1010 (toll free)
Fax: 301-480-9793

Electronic Mail:prpl@mail.cc.nih.gov

Citations:
The antidepressant rolipram suppresses cytokine production and prevents autoimmune encephalomyelitis

Supression of IL-12 production by phosphodiesterase inhibition in murine endotoxemia is IL-10 independent

The immunomodulatory profile of phosphodiesterase type 4-specific inhibition of human autoreactive T cell clones: Basis for the treatment of T helper 1 lymphocytes-mediated autoimmune diseases





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Wise Young
08-15-2004, 03:13 AM
This study has been completed (April 2004). The resultls have not yet been published.

http://clinicaltrials.gov/ct/gui/c/a1b/show/NCT00011375?order=38&JServSessionIdzone_ct=zhn5b9gan1