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Max
04-14-2003, 05:21 PM
Leaving injury behind
Research center at UC Irvine is among the leaders into finding treatment for spinal cord injuries. Supporters include actor Christopher Reeve and heiress Joan Irvine Smith.
a d v e r t i s e m e n t




By Deirdre Newman
Daily Pilot

April 13, 2003

The spinal cord is the body's version of Grand Central Station, a hub of constantly percolating activity, sending impulses to the brain and receiving impulses from it to direct to other parts of the body.

A spinal cord injury can bring this hub to a grinding halt, rendering victims paralyzed throughout their bodies, depending on where the injury occurs.

As treatment for such injuries evolves, minor breakthroughs are leading the charge toward major advancements. At the forefront of the most cutting-edge advanced work is UC Irvine, where one center has positioned itself as a catalyst of research for treating injury and disease of the spinal cord -- conducting a slew of projects in-house while collaborating with leading researchers in the field, scientists outside of the field and spinal cord injury victims.

The goal of the Reeve-Irvine Research Center is to engage this diverse group of researchers in solving one of the trickiest challenges of modern science.

"Spinal cord injuries are immensely complex," said Maura Hofstadter, director of education for the center. "It's like a puzzle and everyone is working on their own little piece. What we're trying to be here is a hub and help bring the puzzle pieces together."

The center evolved out of admiration for Christopher Reeve by Joan Irvine Smith. Smith, an heir to the Irvine development and a horsewoman who owns two ranches, was impressed that Reeve never blamed his horse after being thrown from it during a competition in 1995 that left him paralyzed. Smith offered a matching grant of $1 million to start the center.

"I was very, very grateful, not only for her generosity, but for the reasoning behind it," Reeve said.

In 1999, Oswald Steward was recruited to head the center. Steward, who has a doctorate in psychobiology from UC Irvine, made the cross-country trek from the University of Virginia, where he was chairman of its Department of Neuroscience.

Steward is also the chairman of the Scientific Advisory Committee for the Christopher Reeve Paralysis Foundation, which Reeve and wife, Dana, established.

Reeve said he is impressed with Steward's expeditious approach to research.

"Scientists like Oz Steward believe that the mission is to solve a particular problem as quickly as possible and then move on to something else and as a patient advocate, I of course, appreciate scientists like Ozzie Steward and his team at UCI that share that philosophy that research is not an end in itself," Reeve said. "It is a tool to produce results and the result that we want is to relieve the suffering of people who are paralyzed."

Research on

the cutting edge

The 6,000-square-foot center is housed in the Gillespie Neuroscience Research Center at the College of Medicine and employs about 50 people.

The center encourages collaboration, cooperation and communication and the layout is a testament to that philosophy, Hofstadter said. It is an open space that promotes a free-flowing exchange of ideas, in contrast to most labs that feature long quiet halls and locked doors.

Researchers use mice and rats to study nerve regeneration since they breed rapidly and their genetics are well understood, Hofstadter said. The tiny brains and delicate, thread-like spinal cords of these animals are suspended in solutions in a freezer.

There are three primary researchers -- Steward, Aileen Anderson and Hans Keirstead -- as well as graduate students, post-doctoral students, visiting faculty and undergrads.

"We want to bring students in to pique their interest so they will pursue spinal-cord injury [research]," Hofstadter said.

In addition to working with rats and mice, the center works with human embryonic stem cells.

Keirstead, 35, an assistant professor of anatomy and neurobiology, has access to five of the 63 human embryonic stem cells approved for use in the country.

"What we have done for the first time in the world is to differentiate the human embryonic stem cells into a specialized sub-brain cell type thought to be necessary for repair," Hofstadter said.

After they are differentiated, they are transferred into animals with spinal cord injuries.

The results have been extraordinary, Keirstead said.

Keirstead has already given two Senate pitches for funding of this type of research. He stressed the importance of federal legislators hearing about successful outcomes during their terms of office on research some have supported.

The center is also one of the few labs in the world that has obtained pure lines of human olfactory ensheathing neuroglia -- obtained from the nose -- that have shown potential for cell repair as well, Keirstead said. Neuroglial tissue comprises the bulk of cells in the central nervous system and provide support for the nerve cells.

Preliminary studies have shown that paralyzed animals can walk again after receiving these cells, Keirstead said.

Reaching out for help

In addition to the three main researchers, the center now has 14 faculty members as associates. Because UCI boasts a committed group of neuroscience researchers, Steward wanted to pick their brains on the topic of spinal cord injury.

One of the associates is Sue Bryant, who studies salamander limb regeneration. Another one is Anne Calof, who works with the development of the nervous system in humans.

"If we can figure out how we did it the first time, maybe we can trick the body into doing it again," Hofstadter said.

Aerospace engineer David Reinkensmeyer will soon have a space in nearby Hewitt Hall to work on creating robots to help spinal cord injury victims relearn how to walk. The robots will replace physical therapists for hip and ankle support.

"When we do clinical trials with any of these treatments, rehabilitation will be part of it," Hofstadter said. "We're very excited about it."

Steward also took the initiative to invite the California Spinal Cord Injury Neural Regeneration Consortium, a group of about 100 scientists, to work on solving the puzzle. California has the dubious distinction of producing the highest rate of spinal cord injuries in the country, mostly from car accidents and surfing.

The center makes it a priority to invite feedback from the community it was established to help. It conducts surveys asking spinal cord injury victims what they would like to see the center focus on. Based on the outreach, researchers found that most of what they were emphasizing -- helping patients walk again -- is not the foremost desire. Bladder control is, they found, and added that to their research, Hofstadter said.

It also hosts a meet and greet with the scientists, where spinal cord injury victims, their families and their caregivers can talk to researchers from around the state.

And it has one other source with intimate knowledge of life after an injury. One of the center's own researchers, Kim Anderson, 31, was injured in a car accident in Texas when she was 17 that rendered her a quadriplegic. Her post-doctoral work at the center focuses on how molecules are affected after a cervical spinal cord injury.

"I'm very driven to find treatments of any type to help people with spinal cord injuries, especially quadriplegics, which I am, because we have the biggest burden physically, financially and emotionally," Anderson said. "And I think that people with spinal cord injuries have a great amount of information that they can give researchers about what humans are really going through and can really help the development of looking at outcomes when they're looking at clinical trials."

Backing risky research

In 2000, the president of the University of California asked the center to administer the California Spinal Cord Injury Research Fund (previously the Roman Reed Bill). The Reed bill, named for Roman Reed, a Bay Area man who suffered an paralyzing accident while playing college football, is state-funded program that provides $1 million a year for spinal cord injury research. The center was entrusted with developing a research program for faculty from the UC system and the rest of California.

In line with its philosophy of attacking the puzzle from all angles, the center disburses seed money for projects that might be considered too uncertain by others, Hofstadter said.

"It's a little risky, but we'll get good information, even if [the project] fails," Hofstadter said.

The center gives a smaller amount of funds to the Roman Reed Core Laboratory, which is part of the center. The goal of the lab is to expand the number of people participating in spinal cord injury research. To that end, it invites researchers with an interest in spinal cord injury but who are not working in the field.

"Like a chemist who doesn't know a rat from a dog and doesn't know where the spinal cord is, but wants to explore," Hofstadter said. "We tell them to write up a proposal, then come play with us. So a project that would likely never happen or take years [can happen] in three months. We're fast-tracking ideas."

The center is currently in a campaign to raise $2 million for developing human spinal cord injury studies and treatments and is about three-quarters of the way there, Steward said.

"We're just really excited about what's going on," Steward said. "It's still a unique resource in the world."

* DEIRDRE NEWMAN covers Costa Mesa and may be reached at (949) 574-4221 or by e-mail at deirdre.newman@latimes.com.

http://www.latimes.com/news/local/pilot/features/la-dpt-sundaystory13apr13,1,1913600.story

Max
07-02-2003, 04:53 PM
Stem cell breakthrough gives hope to spinal injury victims

Cells from human embryos have been used to make paralysed rats walk again, it has been revealed.

Scientists say human trials involving people with spinal injuries could start in just two years.

They hope the work will prove to policymakers that controversial research on human embryonic stem cells and therapeutic cloning is justified.

Researchers in the United States first took stem cells from early-stage human embryos and transformed them in the laboratory.

The cells were turned into oligodenrocytes, cells that form myelin, the vital insulating layer that surrounds nerve fibres.

These were transplanted into paralysed rats with bruised spines. After nine weeks, the rats fully regained the ability to walk, New Scientist magazine reports.

Dr Hans Keirstead and his team from the University of California at Irvine now plan to use the same technique to treat human patients who have sustained recent spinal cord injuries and localised damage.

Treating people who have been paralysed for years or suffer from degenerative nerve diseases will be far more difficult.

Stem cells are the body's 'master cells' that can be stimulated to form different kinds of tissue.

Those taken from pinhead-sized early embryos have the potential to become any part of the body, opening up the possibility of revolutionary new treatments.

Therapeutic cloning could allow patients to be treated with embryonic stem cells derived from their own bodies, thus avoiding problems of rejection.

But such research raises serious ethical issues. Britain has allowed scientists to conduct embryonic stem cell experiments, but they could soon be banned by the European Union.


Story filed: 19:43 Wednesday 2nd July 2003

http://www.ananova.com/news/story/sm_796151.html

Max
07-02-2003, 06:34 PM
Originally posted by wcrabtex:

Max,
I suspect this is the info Okarma referred to at the Biotech conference in Washington DC.Thanks for posting.

WCR

Thanks WCR,

I try not to doble post here by searching for key word..

On this occasion it was "Dr Hans Keirstead "

Thats why I posted it here not in separate topic

paulsask
07-02-2003, 06:48 PM
Greetings

The group at Irvine are very focused on sci treatments, unlike the "puppymakers" at the Miami Project.
Hans Keirstead is extremely talented and very pro-cure. He worked a few years at CORD in B.C until he was lured to Irvine with $$$ and state of the art equipment.
It was a good move for him, but a loss for CORD.
I just wonder if they want human application to get underway as fast as we do.
Have any of you people in California ever been to this facility ?
Has Dr. Young been there ?
I bet it would be facsinating to take a tour.

Paul

Max
07-04-2003, 05:50 PM
Hope for spinal injury patients as paralysed rats walk after stem cell transplants
The Irish Examiner 04 Jul 2003



By Sue O'Reilly

NERVE cells from human embryonic stem cells and transplanted into paralysed rats have enabled the animals to walk again. The findings, published yesterday in the New Scientist magazine, add to a growing number of studies that suggest embryonic stem cells could have a valuable role to play in treating spinal injuries.

The researchers, whose work was funded by stem cell giant Geron of Menlo Park, California, say trials on people could start in just two years.

But the first trials are likely to involve patients with recent spinal cord injuries and localised damage. Treating people who have been paralysed for years or suffer from degenerative nerve diseases would be far more difficult.

Ways will also have to be found to prevent people rejecting the stem cells. One possible alternative to immunosuppressant drugs, Geron president Thomas Okarma told the BIO 2003 conference in Washington DC last week, would be to first give patients bone marrow stem cells from the same source as the nerve cells. This might trick the patients' immune system into developing tolerance.

Researchers are exploring a number of approaches to repairing damaged spines, including drugs that overcome spinal cells' reluctance to re-grow, ways of bridging the gap between severed nerves and transplants of various tissues, including adult stem cells derived from bone marrow and nerve cells from the nose. Human trials of some treatments, such as using nose cells, have already begun.

But Okarma thinks adult cells have serious limitations as a mass-market treatment, because not many cells can be grown from a single source. That is not a problem with embryonic stem cells (ESCs). "One cell bank derived from a single embryo produces enough neurons to treat 10 million Parkinson's disease patients," he says.

He claims adult stem cells may not be as versatile. "At this moment, there is very little hard evidence that a bone marrow stem cell can turn into anything but blood or that a skin stem cell can become anything but skin."

ESCs, on the other hand, have the potential to develop into practically any type of tissue. But there is also a serious problem with ESCs.

"Undifferentiated human embryonic stem cells have a very high probability of forming tumours," says Hans Keirstead at the University of California, Irvine, whose team did the latest research.

To prevent this, his team turned ESCs into specialised cells before transplanting them. They transformed the ESCs into oligodendrocytes, cells that form the insulating layer of myelin that is vital for conducting nerve impulses.

Keirstead's team transplanted the oligodendrocytes into rats with "bruised" spines. After nine weeks, the rats fully regained the ability to walk, he says, whereas rats given no therapy remained paralysed.

While many promising spinal repair experiments have proved hard to reproduce, researchers at Johns Hopkins University in Baltimore, Maryland, also announced similar results last week. The team injected undifferentiated human ESCs into rats with injured spinal cords. After 24 weeks, treated rats could support their own weight.




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