Wise Young
04-25-2004, 12:40 AM
• Deumens R, Koopmans GC, Den Bakker CG, Maquet V, Blacher S, Honig WM, Jerome R, Pirard JP, Steinbusch HW and Joosten EA (2004). Alignment of glial cells stimulates directional neurite growth of CNS neurons in vitro. Neuroscience. 125: 591-604. Department of Psychiatry and Neuropsychology, Div. Cellular Neuroscience, European Graduate School for Neuroscience (EURON), Universiteit Maastricht, P.O. Box 616, 6200 MD, Maastricht, The Netherlands. Olfactory ensheathing cells (OECs) together with olfactory nerve fibroblasts (ONFs) and neonatal astrocytes are potent stimulators of neurite growth in adulthood and during development, respectively. Since it is known that alignment of glial cells is important for the correct outgrowth of axon tracts, it was hypothesized that the alignment of glial cells stimulates directional and enhanced neurite outgrowth. Adult OEC/ONF and neonatal astrocytes were cultured either on biodegradable poly(d,l)-lactide matrices or in Petri dishes for 4 days. Thereafter neonatal cerebral cortical neurons were added. After a 2-days coculture period the cultures were fixed and processed for a combined MAP-2 and phosphorylated neurofilament (RT97) staining. The neurite growth (neurite elongation and neurite formation) and the neurite direction were assessed. We show that (1) OEC/ONF cultures are more potent in stimulating the length of the longest neurite of cocultured neurons, (2) alignment of glial is achieved in vitro on our biomatrices, (3) aligned glial/biomatrix complexes do not enhance neurite growth, and (4) aligned glial/biomatrix complexes direct neurite outgrowth. These data have significant implications for in vivo experiments focusing on glial transplantation. Transplanting glial/biomatrix complexes may stimulate the directional regrowth of severed axons across a lesion site.